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Phosphoproteomic Approaches to Evaluate ABA Signaling

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Abscisic Acid

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2462))

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Abstract

Abscisic acid (ABA) is a major phytohormone that regulates various processes in plants (e.g., seed dormancy/germination, abiotic/biotic stress responses). As protein phosphorylation is involved in the major pathways of ABA signaling, it is necessary to elucidate the phosphosignaling pathway involved in the ABA response. Phosphoproteomics enables determination of the proteins phosphorylated in vivo, and recent studies have applied a comparative phosphoproteomic approach to analyze ABA signaling in plants. For example, ABA-responsive phosphoproteins were identified in barley embryos. Furthermore, a phosphoproteomic approach is useful for screening protein kinase substrates by comparative analysis using kinase knockout mutants. Here, some technical points regarding phosphoproteomic analyses of ABA responses in plants are described.

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Acknowledgments

This work was supported by the Japan Science and Technology Agency program PRESTO and Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan

    Kota Yamashita & Taishi Umezawa

  2. Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan

    Taishi Umezawa

Authors
  1. Kota Yamashita
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  2. Taishi Umezawa
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Corresponding author

Correspondence to Taishi Umezawa .

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Editors and Affiliations

  1. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany

    Takuya Yoshida

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Yamashita, K., Umezawa, T. (2022). Phosphoproteomic Approaches to Evaluate ABA Signaling. In: Yoshida, T. (eds) Abscisic Acid. Methods in Molecular Biology, vol 2462. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2156-1_13

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  • DOI: https://doi.org/10.1007/978-1-0716-2156-1_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2155-4

  • Online ISBN: 978-1-0716-2156-1

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