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Reconstitution System of NLRP3 Inflammasome in HEK293T Cells

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The Inflammasome

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2459))

Abstract

NLRP3 (NOD-, LRR-, and pyrin domain-containing protein 3) inflammasome is a cytosolic multimeric protein complex that plays key roles in the host innate immune response to both pathogenic and sterile insults. Here we describe a comprehensive guide to study NLRP3 inflammasome activation in HEK293T cell reconstitution system, which could provide direct biochemical evidence in protein interaction and posttranslational modification of the complex.

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Author information

Authors and Affiliations

  1. Institute of Immunology, and Department of Orthopaedic Surgery of the Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of China

    Sheng Chen, Zhexu Chi & Di Wang

  2. Department of Colorectal Surgery and Oncology, Key Laboratory of Cancer Prevention and Intervention, Ministry of Education, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of China

    Sheng Chen

Authors
  1. Sheng Chen
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  2. Zhexu Chi
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  3. Di Wang
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Corresponding author

Correspondence to Di Wang .

Editor information

Editors and Affiliations

  1. School of Kinesiology and Health Science, York University, Toronto, ON, Canada

    Ali A. Abdul-Sater

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Chen, S., Chi, Z., Wang, D. (2022). Reconstitution System of NLRP3 Inflammasome in HEK293T Cells. In: Abdul-Sater, A.A. (eds) The Inflammasome. Methods in Molecular Biology, vol 2459. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2144-8_8

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  • DOI: https://doi.org/10.1007/978-1-0716-2144-8_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2143-1

  • Online ISBN: 978-1-0716-2144-8

  • eBook Packages: Springer Protocols

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