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Multi-Angle In Vivo Imaging of the Arabidopsis thaliana Shoot Apical Meristem (SAM)

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Plasmodesmata

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2457))

Abstract

Fluorescent imaging, especially in living tissue, has become a key method in modern life sciences, with the development of new tools for sample preparation, imaging, and data analysis continuously advancing our understanding of biological principles. Here, we present our strategy for in vivo imaging of the Arabidopsis shoot apical meristem (SAM), a central structure in plant development. We implement simplifications to previously published workflows and present a novel approach to subsequentially image the meristem from multiple angles at high resolution. This tool may represent a valuable resource for shoot meristem-centered research in general, but also for studies on plasmodesmata or intercellular connectivity within the SAM: via the analysis of fluorescently labeled plasmodesmata-localized proteins, via the tracing of fluorescent dyes, via analyzing the cell-to-cell mobility of fluorescently labeled proteins, but also via the analysis of morphological features of meristematic cells in mutants or upon perturbation of symplastic connectivity.

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Authors and Affiliations

  1. Department of Stem Cell Biology, Centre for Organismal Studies (COS), Heidelberg University, Heidelberg, Germany

    Michael Fuchs & Jan U. Lohmann

Authors
  1. Michael Fuchs
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  2. Jan U. Lohmann
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Corresponding author

Correspondence to Jan U. Lohmann .

Editor information

Editors and Affiliations

  1. Centre for Plant Sciences, School of Biology, University of Leeds, Leeds, UK

    Yoselin Benitez-Alfonso

  2. Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg, Strasbourg, France

    Manfred Heinlein

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Fuchs, M., Lohmann, J.U. (2022). Multi-Angle In Vivo Imaging of the Arabidopsis thaliana Shoot Apical Meristem (SAM). In: Benitez-Alfonso, Y., Heinlein, M. (eds) Plasmodesmata. Methods in Molecular Biology, vol 2457. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2132-5_29

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  • DOI: https://doi.org/10.1007/978-1-0716-2132-5_29

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2131-8

  • Online ISBN: 978-1-0716-2132-5

  • eBook Packages: Springer Protocols

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