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Extraction of Exosomes and Exosomal miRNA from Mesenchymal Stem Cells

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Non-Alcoholic Steatohepatitis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2455))

Abstract

Exosomes, derived from stem cells, have great promise in regenerative medicine due to their capabilities of ameliorating inflammation, preventing tissue damage and promoting healing, which in part are associated with the exosomal RNA/miRNA. The application of mesenchymal stem cell exosomes in treating hepatic disorders including nonalcoholic fatty liver disease has drawn much attention. In this chapter, we describe our experience in culturing human mesenchymal stem cells and isolating their exosomes from culture medium through ultracentrifugation. Methods to extract exosomal RNA/miRNA are also discussed.

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Acknowledgments

This work was supported in part NIH/NIDCR R03DE027146 to ZL.

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Authors and Affiliations

  1. Department of Periodontics, School of Dentistry, Virginia Commonwealth University, Richmond, VA, USA

    Zhao Lin, Andrew Wong & Shiekh Alam

Authors
  1. Zhao Lin
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  2. Andrew Wong
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  3. Shiekh Alam
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Corresponding author

Correspondence to Zhao Lin .

Editor information

Editors and Affiliations

  1. Department of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, VA, USA

    Devanand Sarkar

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Lin, Z., Wong, A., Alam, S. (2022). Extraction of Exosomes and Exosomal miRNA from Mesenchymal Stem Cells. In: Sarkar, D. (eds) Non-Alcoholic Steatohepatitis. Methods in Molecular Biology, vol 2455. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2128-8_25

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  • DOI: https://doi.org/10.1007/978-1-0716-2128-8_25

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2127-1

  • Online ISBN: 978-1-0716-2128-8

  • eBook Packages: Springer Protocols

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