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Single Cell RNA Sequencing in NASH

Part of the Methods in Molecular Biology book series (MIMB,volume 2455)


Single cell RNA sequencing (scRNA-seq) allows to uncover cellular heterogeneity and the identification of novel subpopulations. In non-alcoholic steatohepatitis (NASH), scRNA-seq is particularly powerful to understand non-parenchymal cell heterogeneity in the liver, e.g. for inflammatory cells. Myeloid immune cells, particularly macrophages, play a critical role in response of the innate immune system and significantly contribute to the progression of fatty liver disease. Due to their high heterogeneity and complex phenotypes, their functional role in health and disease is difficult to analyze. Here, we describe the isolation and analysis of myeloid cell populations from mouse liver using microdroplet-based scRNA-seq. This approach allows the identification and characterization of different hepatic cell types, exemplified here by hepatic macrophage populations, as well as analyses of differentially expressed genes between samples (e.g., cells from healthy or NASH livers).

Key words

  • Single cell RNA sequencing (scRNA-seq)
  • NASH
  • Myeloid immune cells
  • 10× Genomics
  • Macrophages
  • Fibrosis
  • Transcriptomics

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  • DOI: 10.1007/978-1-0716-2128-8_15
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This work was supported by the German Research Foundation (DFG, SFB/TRR296, CRC1382, Ta434/3-1 and Ta434/5-1).

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Correspondence to Frank Tacke .

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Hundertmark, J., Berger, H., Tacke, F. (2022). Single Cell RNA Sequencing in NASH. In: Sarkar, D. (eds) Non-Alcoholic Steatohepatitis. Methods in Molecular Biology, vol 2455. Humana, New York, NY.

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2127-1

  • Online ISBN: 978-1-0716-2128-8

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