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Culture of Mouse Liver Ductal Organoids

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Part of the Methods in Molecular Biology book series (MIMB,volume 2455)


3D organoid culture has become a powerful tool and model for various human diseases, including liver diseases, such as non-alcoholic steatohepatitis (NASH). Hepatic organoids have significant advantages over traditional primary cell cultures. The hepatic progenitor cells can be induced to form hepatic organoids. The established organoids can be passaged or cryopreserved for future use. The established hepatic organoids can be manipulated to study the disease progression of NASH-related fibrosis. Here, we describe a protocol to establish mouse liver ductal organoids.

Key words

  • Organoids
  • Bile duct
  • Liver
  • Hepatocytes
  • Cholangiocytes

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  • DOI: 10.1007/978-1-0716-2128-8_11
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We thank Mrs. Elaine Kennedy for proofreading and English editing. This work was supported by VA Merit Awards I01BX004033; Research Career Scientist Award IK6BX004477; National Institutes of Health Grants (R01 DK104893, R01DK-057543, and R21 AA026629-01).

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Correspondence to Huiping Zhou .

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Yan, J., Tai, Y., Zhou, H. (2022). Culture of Mouse Liver Ductal Organoids. In: Sarkar, D. (eds) Non-Alcoholic Steatohepatitis. Methods in Molecular Biology, vol 2455. Humana, New York, NY.

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2127-1

  • Online ISBN: 978-1-0716-2128-8

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