Abstract
Bottom-up proteomics enables a systems-level analysis of proteins involved in a particular sample set. In this protocol, we describe the workflow to prepare Klebsiella pneumoniae and macrophage cells for co-culture, how to extract and prepare samples for analysis by high-resolution mass spectrometry, and lastly, how to analyze the output data files. This workflow allows for the identification of proteins involved in both the bacterial and host perspective during pathogenesis.
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Sukumaran, A., Geddes-McAlister, J. (2022). Proteomic Profiling of the Interplay Between a Bacterial Pathogen and Host Uncovers Novel Anti-Virulence Strategies. In: Geddes-McAlister, J. (eds) Proteomics in Systems Biology. Methods in Molecular Biology, vol 2456. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2124-0_17
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DOI: https://doi.org/10.1007/978-1-0716-2124-0_17
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