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SARS-CoV-2 pp 183–196Cite as

Detection of SARS-CoV-2 by Mass Spectrometry

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Part of the Methods in Molecular Biology book series (MIMB,volume 2452)


Amplification of viral ribonucleic acid by real-time reverse transcriptase polymerase chain reaction is the gold standard to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, alternative reliable, fast, and cost-effective methods for the detection of SARS-CoV-2 are still needed. In this chapter, the mass spectrometry–based detection of amplified polymerase chain reaction products of SARS-CoV-2 genes from oral or nasopharyngeal swabs is described. The respective SARS-CoV-2 test has previously been shown to meet standard quality criteria and was therefore approved by the authorities in Europe and the USA.

Key words

  • Mass Spectrometry
  • MassARRAY
  • SARS-CoV-2
  • COVID-19

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  • DOI: 10.1007/978-1-0716-2111-0_12
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  1. Guan WJ, Ni ZY, Hu Y, Liang WH, Ou CQ, He JX, Liu L, Shan H, Lei CL, DSC H, Du B, Li LJ, Zeng G, Yuen KY, Chen RC, Tang CL, Wang T, Chen PY, Xiang J, Li SY, Wang JL, Liang ZJ, Peng YX, Wei L, Liu Y, Hu YH, Peng P, Wang JM, Liu JY, Chen Z, Li G, Zheng ZJ, Qiu SQ, Luo J, Ye CJ, Zhu SY, Zhong NS, China Medical Treatment Expert Group for C (2020) Clinical characteristics of coronavirus disease 2019 in China. N Engl J Med 382(18):1708–1720.

    CAS  CrossRef  PubMed  Google Scholar 

  2. Baden LR, El Sahly HM, Essink B, Kotloff K, Frey S, Novak R, Diemert D, Spector SA, Rouphael N, Creech CB, McGettigan J, Kehtan S, Segall N, Solis J, Brosz A, Fierro C, Schwartz H, Neuzil K, Corey L, Gilbert P, Janes H, Follmann D, Marovich M, Mascola J, Polakowski L, Ledgerwood J, Graham BS, Bennett H, Pajon R, Knightly C, Leav B, Deng W, Zhou H, Han S, Ivarsson M, Miller J, Zaks T, Group CS (2020) Efficacy and safety of the mRNA-1273 SARS-CoV-2 vaccine. N Engl J Med.

  3. Polack FP, Thomas SJ, Kitchin N, Absalon J, Gurtman A, Lockhart S, Perez JL, Perez Marc G, Moreira ED, Zerbini C, Bailey R, Swanson KA, Roychoudhury S, Koury K, Li P, Kalina WV, Cooper D, Frenck RW Jr, Hammitt LL, Tureci O, Nell H, Schaefer A, Unal S, Tresnan DB, Mather S, Dormitzer PR, Sahin U, Jansen KU, Gruber WC, Group CCT (2020) Safety and efficacy of the BNT162b2 mRNA Covid-19 vaccine. N Engl J Med 383(27):2603–2615.

    CAS  CrossRef  PubMed  Google Scholar 

  4. Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, Bleicker T, Brunink S, Schneider J, Schmidt ML, Mulders DG, Haagmans BL, van der Veer B, van den Brink S, Wijsman L, Goderski G, Romette JL, Ellis J, Zambon M, Peiris M, Goossens H, Reusken C, Koopmans MP, Drosten C (2020) Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill 25(3).

  5. Mascuch SJ, Fakhretaha-Aval S, Bowman JC, Ma MTH, Thomas G, Bommarius B, Ito C, Zhao L, Newnam GP, Matange KR, Thapa HR, Barlow B, Donegan RK, Nguyen NA, Saccuzzo EG, Obianyor CT, Karunakaran SC, Pollet P, Rothschild-Mancinelli B, Mestre-Fos S, Guth-Metzler R, Bryksin AV, Petrov AS, Hazell M, Ibberson CB, Penev PI, Mannino RG, Lam WA, Garcia AJ, Kubanek J, Agarwal V, Hud NV, Glass JB, Williams LD, Lieberman RL (2020) A blueprint for academic laboratories to produce SARS-CoV-2 quantitative RT-PCR test kits. J Biol Chem 295(46):15438–15453.

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  6. Klein S, Muller TG, Khalid D, Sonntag-Buck V, Heuser AM, Glass B, Meurer M, Morales I, Schillak A, Freistaedter A, Ambiel I, Winter SL, Zimmermann L, Naumoska T, Bubeck F, Kirrmaier D, Ullrich S, Barreto Miranda I, Anders S, Grimm D, Schnitzler P, Knop M, Krausslich HG, Dao Thi VL, Borner K, Chlanda P (2020) SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP. Viruses 12(8).

  7. Seger C, Salzmann L (2020) After another decade: LC-MS/MS became routine in clinical diagnostics. Clin Biochem.

  8. Borden SA, Palaty J, Termopoli V, Famiglini G, Cappiello A, Gill CG, Palma P (2020) Mass spectrometry analysis of drugs of abuse: challenges and emerging strategies. Mass Spectrom Rev.

  9. Fung AWS, Sugumar V, Ren AH, Kulasingam V (2020) Emerging role of clinical mass spectrometry in pathology. J Clin Pathol 73(2):61–69.

    CAS  CrossRef  PubMed  Google Scholar 

  10. Angeletti S, Ciccozzi M (2019) Matrix-assisted laser desorption ionization time-of-flight mass spectrometry in clinical microbiology: an updating review. Infect Genet Evol 76:104063.

    CAS  CrossRef  PubMed  Google Scholar 

  11. De Roock W, Claes B, Bernasconi D, De Schutter J, Biesmans B, Fountzilas G, Kalogeras KT, Kotoula V, Papamichael D, Laurent-Puig P, Penault-Llorca F, Rougier P, Vincenzi B, Santini D, Tonini G, Cappuzzo F, Frattini M, Molinari F, Saletti P, De Dosso S, Martini M, Bardelli A, Siena S, Sartore-Bianchi A, Tabernero J, Macarulla T, Di Fiore F, Gangloff AO, Ciardiello F, Pfeiffer P, Qvortrup C, Hansen TP, Van Cutsem E, Piessevaux H, Lambrechts D, Delorenzi M, Tejpar S (2010) Effects of KRAS, BRAF, NRAS, and PIK3CA mutations on the efficacy of cetuximab plus chemotherapy in chemotherapy-refractory metastatic colorectal cancer: a retrospective consortium analysis. Lancet Oncol 11(8):753–762.

    CAS  CrossRef  PubMed  Google Scholar 

  12. Wandernoth P, Kriegsmann K, Groh-Mohanu C, Daeumer M, Gohl P, Harzer O, Kriegsmann M, Kriegsmann J (2020) Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by mass spectrometry. Viruses 12(8).

  13. Administration FaD (2020) Fact sheet for healthcare providers: massarray SARS-CoV-2 panel

    Google Scholar 

  14. Mifflin TE (2007) Setting up a PCR laboratory. CSH Protoc 2007:pdb top14.

  15. Kriegsmann M, Wandernoth P, Lisenko K, Casadonte R, Longuespee R, Arens N, Kriegsmann J (2017) Detection of HPV subtypes by mass spectrometry in FFPE tissue specimens: a reliable tool for routine diagnostics. J Clin Pathol 70(5):417–423.

    CAS  CrossRef  PubMed  Google Scholar 

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Correspondence to Mark Kriegsmann .

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Wandernoth, P., Kriegsmann, K., Kriegsmann, J., Kriegsmann, M. (2022). Detection of SARS-CoV-2 by Mass Spectrometry. In: Chu, J.J.H., Ahidjo, B.A., Mok, C.K. (eds) SARS-CoV-2. Methods in Molecular Biology, vol 2452. Humana, New York, NY.

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2110-3

  • Online ISBN: 978-1-0716-2111-0

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