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SARS-CoV-2 pp 183–196Cite as

Detection of SARS-CoV-2 by Mass Spectrometry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2452))

Abstract

Amplification of viral ribonucleic acid by real-time reverse transcriptase polymerase chain reaction is the gold standard to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, alternative reliable, fast, and cost-effective methods for the detection of SARS-CoV-2 are still needed. In this chapter, the mass spectrometry–based detection of amplified polymerase chain reaction products of SARS-CoV-2 genes from oral or nasopharyngeal swabs is described. The respective SARS-CoV-2 test has previously been shown to meet standard quality criteria and was therefore approved by the authorities in Europe and the USA.

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Author information

Authors and Affiliations

  1. Molecular Pathology Trier, Trier, Germany

    Petra Wandernoth & Jörg Kriegsmann

  2. Department of Hematology, Oncology and Rheumatology, University Hospital Heidelberg, Heidelberg, Germany

    Katharina Kriegsmann

  3. Danube Private University Krems, Krems, Austria

    Jörg Kriegsmann

  4. Proteopath Trier, Trier, Germany

    Jörg Kriegsmann

  5. Institute of Pathology, University Hospital Heidelberg, Heidelberg, Germany

    Mark Kriegsmann

  6. Member of the German Centre for Lung Research (DZL), Translational Lung Research Centre Heidelberg, Heidelberg, Germany

    Mark Kriegsmann

Authors
  1. Petra Wandernoth
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  2. Katharina Kriegsmann
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  3. Jörg Kriegsmann
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  4. Mark Kriegsmann
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Corresponding author

Correspondence to Mark Kriegsmann .

Editor information

Editors and Affiliations

  1. Department of Microbiology and Immunology; Infectious Disease TRD; BSL3 Core Facility, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

    Justin Jang Hann Chu

  2. The University of British Columbia, Vancouver, BC, Canada

    Bintou Ahmadou Ahidjo

  3. BSL3 Core Facility, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

    Chee Keng Mok

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Cite this protocol

Wandernoth, P., Kriegsmann, K., Kriegsmann, J., Kriegsmann, M. (2022). Detection of SARS-CoV-2 by Mass Spectrometry. In: Chu, J.J.H., Ahidjo, B.A., Mok, C.K. (eds) SARS-CoV-2. Methods in Molecular Biology, vol 2452. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2111-0_12

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  • DOI: https://doi.org/10.1007/978-1-0716-2111-0_12

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2110-3

  • Online ISBN: 978-1-0716-2111-0

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