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Signaling Lipidomic Analysis of Thermogenic Adipocytes

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Brown Adipose Tissue

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2448))

Abstract

Brown adipose tissue is a thermogenic organ that possesses anti-diabetic and anti-obesogenic potential. There has recently been growing interest on the secretory role of brown adipose tissue in regulating whole-body metabolism. Several signaling lipids, including 12-HEPE and 12,13-diHOME, have been shown to be secreted by brown adipose tissue and have demonstrated roles in regulating whole-body energy metabolism. Lipidomics platforms that broadly characterize the signaling lipidome can deconvolute the underlying biology of the lipid metabolites having a broad systemic impact on physiology. Herein, we describe how to perform and analyze LC-MS/MS signaling lipidomics on mature brown adipocytes.

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Acknowledgements

This work was supported by the US National Institutes of Health (NIH) grants R01DK077097, R01DK102898, R01DK122808 (to Y.-H.T.), P30DK036836 (to Joslin Diabetes Center’s Diabetes Research Center) and by the US Army Medical Research Grant W81XWH-17-1-0428. S.D.K. was supported by NIH Training grant T32 DK007260.

Declaration of Interests

The following authors declare competing interests: M.A.K. N.R.N and V.B. are employees of BERG.

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Author notes

  1. Sean D. Kodani and Valerie Bussberg contributed equally with all other contributors.

Authors and Affiliations

  1. Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA, USA

    Sean D. Kodani & Yu-Hua Tseng

  2. BERG, Framingham, MA, USA

    Valerie Bussberg, Niven R. Narain & Michael A. Kiebish

Authors
  1. Sean D. Kodani
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  2. Valerie Bussberg
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  3. Niven R. Narain
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  4. Michael A. Kiebish
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  5. Yu-Hua Tseng
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Corresponding authors

Correspondence to Michael A. Kiebish or Yu-Hua Tseng .

Editor information

Editors and Affiliations

  1. Program in Molecular Medicine, UMass Chan Medical School, Worcester, MA, USA

    David A. Guertin

  2. ETH Zürich, Schwerzenbach, Zürich, Switzerland

    Christian Wolfrum

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Kodani, S.D., Bussberg, V., Narain, N.R., Kiebish, M.A., Tseng, YH. (2022). Signaling Lipidomic Analysis of Thermogenic Adipocytes. In: Guertin, D.A., Wolfrum, C. (eds) Brown Adipose Tissue. Methods in Molecular Biology, vol 2448. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2087-8_16

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  • DOI: https://doi.org/10.1007/978-1-0716-2087-8_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2086-1

  • Online ISBN: 978-1-0716-2087-8

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