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Plant Proteases and Plant Cell Death pp 271–283Cite as

A User Guide to Validation, Annotation, and Evaluation of N-Terminome Datasets with MANTI

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Part of the Methods in Molecular Biology book series (MIMB,volume 2447)

Abstract

A large variety of enrichment procedures for protein N-termini have been developed to trace protease activity and determine precise cleavage sites, as well as other N-terminal protein modifications. Typically, enriched N-terminal peptides are identified by tandem mass spectrometry using standard database search engines, in many cases the popular MaxQuant software package. MaxQuant Advanced N-termini Interpreter (MANTI) is a software package that helps to validate, annotate, and visualize peptide identifications in N-termini datasets in a rapid and straightforward manner. Usage of MANTI and especially its graphical interface Yoğurtlu MANTI in detail are described to enable users to take full advantage of the software package and the multitude of options it has to offer.

Key words

  • N-termini
  • N-terminomics
  • Proteolysis
  • Positional proteomics
  • Bioinformatics
  • MANTI
  • Analysis

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Author information

Authors and Affiliations

  1. Central Institute for Engineering, Electronics and Analytics, ZEA-3, Forschungszentrum Jülich, Jülich, Germany

    Fatih Demir & Pitter F. Huesgen

  2. Department of Biomedicine, Aarhus University, Aarhus, Denmark

    Fatih Demir

  3. Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Medical Faculty and University Hospital, University of Cologne, Cologne, Germany

    Pitter F. Huesgen

  4. Department for Chemistry, Institute of Biochemistry, University of Cologne, Cologne, Germany

    Pitter F. Huesgen

Authors
  1. Fatih Demir
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  2. Pitter F. Huesgen
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Corresponding author

Correspondence to Fatih Demir .

Editor information

Editors and Affiliations

  1. Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia

    Marina Klemenčič

  2. VIB-UGent Cntr for Plant Systems Biology, Ghent University, Ghent, Belgium

    Simon Stael

  3. ZEA-3 Analytik, Forschungszentrum Juelich, Juelich, Germany

    Prof. Dr. Pitter F. Huesgen

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Demir, F., Huesgen, P.F. (2022). A User Guide to Validation, Annotation, and Evaluation of N-Terminome Datasets with MANTI. In: Klemenčič, M., Stael, S., Huesgen, P.F. (eds) Plant Proteases and Plant Cell Death. Methods in Molecular Biology, vol 2447. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2079-3_22

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  • DOI: https://doi.org/10.1007/978-1-0716-2079-3_22

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2078-6

  • Online ISBN: 978-1-0716-2079-3

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