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Induction and Detection of Mitophagy

Part of the Methods in Molecular Biology book series (MIMB,volume 2445)

Abstract

Mitophagy, a process of selective elimination of mitochondria by autophagy, is a mechanism of mitochondrial quality control that maintains mitochondrial network functionality. The elimination of damaged mitochondria through autophagy requires two steps: induction of general autophagy and priming of damaged mitochondria for selective autophagic recognition. Mitophagy impairment is linked to various pathologies; thus, removal of malfunctioning or even harmful mitochondria is vital to cellular physiology. Here, we describe methods that can be applied to the investigation of mitophagy.

Key words

  • Mitophagy
  • Autophagy
  • Mitochondria
  • Respiration
  • Confocal microscopy
  • Flow cytometry

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  • DOI: 10.1007/978-1-0716-2071-7_14
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Acknowledgments

This research was funded by the Russian Science Foundation (grant 19-14-00122). The work in the authors’ laboratories was also supported by grants from the Russian Foundation for Basic Research (20-015-00105), Swedish Cancer Society (190345) and Stockholm Cancer Society (181301).

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The authors declare no conflict of interest.

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Correspondence to Vladimir Gogvadze .

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Yapryntseva, M.A., Zhivotovsky, B., Gogvadze, V. (2022). Induction and Detection of Mitophagy. In: Norberg, H., Norberg, E. (eds) Autophagy and Cancer. Methods in Molecular Biology, vol 2445. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2071-7_14

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  • DOI: https://doi.org/10.1007/978-1-0716-2071-7_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2070-0

  • Online ISBN: 978-1-0716-2071-7

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