Skip to main content
SpringerLink
Log in

Induction and Detection of Mitophagy

  • Protocol
  • First Online:
Autophagy and Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2445))

Abstract

Mitophagy, a process of selective elimination of mitochondria by autophagy, is a mechanism of mitochondrial quality control that maintains mitochondrial network functionality. The elimination of damaged mitochondria through autophagy requires two steps: induction of general autophagy and priming of damaged mitochondria for selective autophagic recognition. Mitophagy impairment is linked to various pathologies; thus, removal of malfunctioning or even harmful mitochondria is vital to cellular physiology. Here, we describe methods that can be applied to the investigation of mitophagy.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Nguyen TN, Padman BS, Lazarou M (2016) Deciphering the molecular signals of PINK1/Parkin mitophagy. Trends Cell Biol 26:733–744. https://doi.org/10.1016/j.tcb.2016.05.008

    Article  CAS  PubMed  Google Scholar 

  2. Vara-Perez M, Felipe-Abrio B, Agostinis P (2019) Mitophagy in cancer: a tale of adaptation. Cells 8:493. https://doi.org/10.3390/cells8050493

    Article  CAS  PubMed Central  Google Scholar 

  3. Ibáñez P, Lesage S, Lohmann E et al (2006) Mutational analysis of the PINK1 gene in early-onset parkinsonism in Europe and North Africa. Brain 129:686–694. https://doi.org/10.1093/brain/awl005

    Article  PubMed  Google Scholar 

  4. Kerr JS, Adriaanse BA, Greig NH et al (2017) Mitophagy and Alzheimer’s disease: cellular and molecular mechanisms. Trends Neurosci 40:151–166. https://doi.org/10.1016/j.tins.2017.01.002

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Li X, Huang L, Lan J et al (2021) Molecular mechanisms of mitophagy and its roles in neurodegenerative diseases. Pharmacol Res 163:105240. https://doi.org/10.1016/j.phrs.2020.105240

    Article  CAS  PubMed  Google Scholar 

  6. Luo H, Zhang R, Krigman J et al (2020) A healthy heart and a healthy brain: looking at mitophagy. Front Cell Dev Biol 8:294. https://doi.org/10.3389/fcell.2020.00294

    Article  PubMed  PubMed Central  Google Scholar 

  7. Bernardini JP, Lazarou M, Dewson G (2017) Parkin and mitophagy in cancer. Oncogene 36:1315–1327. https://doi.org/10.1038/onc.2016.302

    Article  CAS  PubMed  Google Scholar 

  8. Wu L, Zhang D, Zhou L et al (2019) FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis. EBioMedicine 41:384–394. https://doi.org/10.1016/j.ebiom.2019.02.032

    Article  PubMed  PubMed Central  Google Scholar 

  9. Hui L, Wu H, Wang T-W et al (2019) Hydrogen peroxide-induced mitophagy contributes to laryngeal cancer cells survival via the upregulation of FUNDC1. Clin Transl Oncol 21:596–606. https://doi.org/10.1007/s12094-018-1958-5

    Article  CAS  PubMed  Google Scholar 

  10. Hou H, Er P, Cheng J et al (2017) High expression of FUNDC1 predicts poor prognostic outcomes and is a promising target to improve chemoradiotherapy effects in patients with cervical cancer. Cancer Med 6:1871–1881. https://doi.org/10.1002/cam4.1112

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Lemasters JJ (2005) Selective mitochondrial autophagy, or mitophagy, as a targeted defense against oxidative stress, mitochondrial dysfunction, and aging. Rejuvenation Res 8:3–5. https://doi.org/10.1089/rej.2005.8.3

    Article  CAS  PubMed  Google Scholar 

  12. Sowter HM, Ratcliffe PJ, Watson P et al (2001) HIF-1-dependent regulation of hypoxic induction of the cell death factors BNIP3 and NIX in human tumors. Cancer Res 61:6669–6673

    CAS  PubMed  Google Scholar 

  13. Mooney RA (1988) [18] Use of digitonin-permeabilized adipocytes for cAMP studies, pp 193–202

    Google Scholar 

  14. Mauro-Lizcano M, Esteban-Martínez L, Seco E et al (2015) New method to assess mitophagy flux by flow cytometry. Autophagy 11:833–843. https://doi.org/10.1080/15548627.2015.1034403

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Jin SM, Lazarou M, Wang C, Kane LA, Narendra DP, Youle RJ. Mitochondrial membrane potential regulates PINK1 import and proteolytic destabilization by PARL. J Cell Biol. 2010 Nov 29;191(5):933-42. doi:10.1083/jcb.201008084. PMID: 21115803; PMCID: PMC2995166.

    Google Scholar 

Download references

Acknowledgments

This research was funded by the Russian Science Foundation (grant 19-14-00122). The work in the authors’ laboratories was also supported by grants from the Russian Foundation for Basic Research (20-015-00105), Swedish Cancer Society (190345) and Stockholm Cancer Society (181301).

Conflict of Interests

The authors declare no conflict of interest.

Author information

Authors and Affiliations

  1. Faculty of Basic Medicine, Lomonosov Moscow State University, Moscow, Russia

    Maria A. Yapryntseva, Boris Zhivotovsky & Vladimir Gogvadze

  2. Karolinska Institutet, Institute of Environmental Medicine, Stockholm, Sweden

    Boris Zhivotovsky & Vladimir Gogvadze

Authors
  1. Maria A. Yapryntseva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Boris Zhivotovsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vladimir Gogvadze
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Vladimir Gogvadze .

Editor information

Editors and Affiliations

  1. Karolinska Institute, Stockholm, Sweden

    Helin Norberg

  2. Karolinska Institute, Stockholm, Sweden

    Erik Norberg

Rights and permissions

Reprints and permissions

Copyright information

© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Yapryntseva, M.A., Zhivotovsky, B., Gogvadze, V. (2022). Induction and Detection of Mitophagy. In: Norberg, H., Norberg, E. (eds) Autophagy and Cancer. Methods in Molecular Biology, vol 2445. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2071-7_14

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-2071-7_14

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2070-0

  • Online ISBN: 978-1-0716-2071-7

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation