Abstract
Examining protein–protein interactions provides critical insight into numerous human diseases and infections. Here we describe a protocol for bimolecular fluorescence complementation, which can be used to directly visualize and characterize intracellular protein-protein interactions and ascertain their localization using fluorescence microscopy.
Key words
- Bimolecular fluorescence complementation
- Protein–protein interactions
- Microscopy
- Subcellular localization
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Acknowledgments
Work in the Dikeakos Lab is supported by a project grant to JDD from the Canadian Institutes of Health Research (CIHR Project Grant 389413) and by a Discovery Grant from the Natural Sciences and Engineering Research Council. CRE is the recipient of a Canadian Institutes of Health Research Canada Graduate Scholarship.
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Edgar, C.R., Dikeakos, J.D. (2022). Bimolecular Fluorescence Complementation to Visualize Protein–Protein Interactions in Cells. In: Heit, B. (eds) Fluorescent Microscopy. Methods in Molecular Biology, vol 2440. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2051-9_5
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DOI: https://doi.org/10.1007/978-1-0716-2051-9_5
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2050-2
Online ISBN: 978-1-0716-2051-9
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