Abstract
Examining protein–protein interactions provides critical insight into numerous human diseases and infections. Here we describe a protocol for bimolecular fluorescence complementation, which can be used to directly visualize and characterize intracellular protein-protein interactions and ascertain their localization using fluorescence microscopy.
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References
Ryan DP, Matthews JM (2005) Protein-protein interactions in human disease. Curr Opin Struct Biol 15:441–446
Wachi S, Yoneda K, Wu R (2005) Interactome-transcriptome analysis reveals the high centrality of genes differentially expressed in lung cancer tissues. Bioinformatics 21:4205–4208
Jonsson PF, Bates PA (2006) Global topological features of cancer proteins in the human interactome. Bioinformatics 22:2291–2297
Chuang HY, Lee E, Liu YT et al (2007) Network-based classification of breast cancer metastasis. Mol Syst Biol 3:140
Goodacre N, Devkota P, Bae E et al (2020) Protein-protein interactions of human viruses. Semin Cell Dev Biol 99:31–39
Hu CD, Chinenov Y, Kerppola TK (2002) Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell 9:789–798
Kerppola TK (2008) Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells. Annu Rev Biophys 37:465–487
Dirk BS, Pawlak EN, Johnson AL et al (2016) HIV-1 Nef sequesters MHC-I intracellularly by targeting early stages of endocytosis and recycling. Sci Rep 6:37021
Prévost J, Edgar CR, Richard J et al (2020) HIV-1 Vpu downregulates Tim-3 from the surface of infected CD4+ T cells. J Virol 94:e01999–e01919
Dirk BS, Jacob RA, Johnson AL et al (2015) Viral bimolecular fluorescence complementation: a novel tool to study intracellular vesicular trafficking pathways. PLoS One 10:e0125619
Acknowledgments
Work in the Dikeakos Lab is supported by a project grant to JDD from the Canadian Institutes of Health Research (CIHR Project Grant 389413) and by a Discovery Grant from the Natural Sciences and Engineering Research Council. CRE is the recipient of a Canadian Institutes of Health Research Canada Graduate Scholarship.
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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Edgar, C.R., Dikeakos, J.D. (2022). Bimolecular Fluorescence Complementation to Visualize Protein–Protein Interactions in Cells. In: Heit, B. (eds) Fluorescent Microscopy. Methods in Molecular Biology, vol 2440. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2051-9_5
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DOI: https://doi.org/10.1007/978-1-0716-2051-9_5
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2050-2
Online ISBN: 978-1-0716-2051-9
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