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Mass Spectrometry Imaging of Small Molecules pp 117–125Cite as

Mass Spectrometry Imaging of Metabolites by Nanostructure Initiator Mass Spectrometry with Fluorinated Gold Nanoparticles

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2437))

Abstract

Nanostructure initiator mass spectrometry (NIMS) with fluorinated gold nanoparticles (f-AuNPs) enables the detection and spatial localization of a breath of polar metabolites and lipids with high spatial resolution and ultrasensitivity. Here we describe the methods and procedures for the synthesis and application of f-AuNPs for NIMS of small molecule metabolites and lipids in biological tissues, encompassing sample preparation, mass spectrometric detection, and data analysis and interpretation.

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Author information

Authors and Affiliations

  1. Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, USA

    Amelia Palermo

Authors
  1. Amelia Palermo
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Correspondence to Amelia Palermo .

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Editors and Affiliations

  1. Department of Chemistry, Iowa State University, Ames, IA, USA

    Young-Jin Lee

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Palermo, A. (2022). Mass Spectrometry Imaging of Metabolites by Nanostructure Initiator Mass Spectrometry with Fluorinated Gold Nanoparticles. In: Lee, YJ. (eds) Mass Spectrometry Imaging of Small Molecules. Methods in Molecular Biology, vol 2437. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2030-4_8

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  • DOI: https://doi.org/10.1007/978-1-0716-2030-4_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2029-8

  • Online ISBN: 978-1-0716-2030-4

  • eBook Packages: Springer Protocols

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