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MALDI-2 and t-MALDI-2 Mass Spectrometry Imaging

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Mass Spectrometry Imaging of Small Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2437))

Abstract

Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) combined with laser-induced postionization for MALDI-2 enables the simultaneous registration of numerous classes of small molecules (e.g., secondary metabolites including sterols) as well as phospholipids, glycolipids, and glycans from tissue sections and from cell cultures with strongly boosted ion yields. Here, we describe methodological aspects that are key for optimizing the analytical sensitivity and spatial resolution of a MALDI-2 imaging experiment. We will include both top-illumination MALDI-2 as well as the recently introduced transmission (t-) mode MALDI-2 approach.

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Acknowledgments

We acknowledge the invaluable input and support from numerous expert colleagues, many of whom are listed as coauthors on the cited papers. Financial support by the German Research Foundation (DFG; grants DR416/9-1, project number 208319078, to K.D.; DR416/12-1 and SO976/3-1, project number 290343045, to K.D. and J.S.; and SO976/5-1, project number 400912714, to J.S.) and the Interdisciplinary Center for Clinical Research (IZKF) of the Münster University Medical School (grant Drei2/018/17, to K.D. and J.S.) is gratefully acknowledged.

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Authors and Affiliations

  1. Institute of Hygiene, University of Münster, Münster, Germany

    Klaus Dreisewerd, Tanja Bien & Jens Soltwisch

Authors
  1. Klaus Dreisewerd
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  2. Tanja Bien
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  3. Jens Soltwisch
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Corresponding author

Correspondence to Klaus Dreisewerd .

Editor information

Editors and Affiliations

  1. Department of Chemistry, Iowa State University, Ames, IA, USA

    Young-Jin Lee

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Dreisewerd, K., Bien, T., Soltwisch, J. (2022). MALDI-2 and t-MALDI-2 Mass Spectrometry Imaging. In: Lee, YJ. (eds) Mass Spectrometry Imaging of Small Molecules. Methods in Molecular Biology, vol 2437. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2030-4_2

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  • DOI: https://doi.org/10.1007/978-1-0716-2030-4_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2029-8

  • Online ISBN: 978-1-0716-2030-4

  • eBook Packages: Springer Protocols

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