Abstract
dropletProbe mass spectrometry is a novel technique for molecular characterization of surfaces. It can be used for rapid ex vivo analysis of therapeutics from thin animal tissue sections and has been shown to improve understanding of a drug’s absorption, distribution, metabolism and excretion (ADME) properties. Here, we describe the tissue distribution analysis of diclofenac from a dosed whole-body mouse thin tissue section using a dropletProbe mass spectrometry system.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Solon EG (2015) Autoradiography techniques and quantification of drug distribution. Cell Tissue Res 360(1):87–107. https://doi.org/10.1007/s00441-014-2093-4
Solon EG, Schweitzer A, Stoeckli M, Prideaux B (2010) Autoradiography, MALDI-MS, and SIMS-MS imaging in pharmaceutical discovery and development. AAPS J 12(1):11–26. https://doi.org/10.1208/s12248-009-9158-4
Buchberger AR, DeLaney K, Johnson J, Li L (2018) Mass spectrometry imaging: a review of emerging advancements and future insights. Anal Chem 90(1):240–265. https://doi.org/10.1021/acs.analchem.7b04733
Duenas ME, Essner JJ, Lee YJ (2017) 3D MALDI mass spectrometry imaging of a single cell: spatial mapping of lipids in the embryonic development of zebrafish. Sci Rep 7(1):14946. https://doi.org/10.1038/s41598-017-14949-x
Norris JL, Caprioli RM (2013) Analysis of tissue specimens by matrix-assisted laser desorption/ionization imaging mass spectrometry in biological and clinical research. Chem Rev 113(4):2309–2342. https://doi.org/10.1021/cr3004295
Aichler M, Walch A (2015) MALDI imaging mass spectrometry: current frontiers and perspectives in pathology research and practice. Lab Investig 95(4):422–431. https://doi.org/10.1038/labinvest.2014.156
Eikel D, Vavrek M, Smith S, Bason C, Yeh S, Korfmacher WA, Henion JD (2011) Liquid extraction surface analysis mass spectrometry (LESA-MS) as a novel profiling tool for drug distribution and metabolism analysis: the terfenadine example. Rapid Commun Mass Spectrom 25(23):3587–3596. https://doi.org/10.1002/rcm.5274
Wu Q, Huang Z, Wang Y, Zhang Z, Lu H (2020) Absolute quantitative imaging of sphingolipids in brain tissue by exhaustive liquid microjunction surface sampling-liquid chromatography-mass spectrometry. J Chromatogr A 1609:460436. https://doi.org/10.1016/j.chroma.2019.460436
Kertesz V, Van Berkel GJ (2010) Liquid microjunction surface sampling coupled with high-pressure liquid chromatography-electrospray ionization-mass spectrometry for analysis of drugs and metabolites in whole-body thin tissue sections. Anal Chem 82(14):5917–5921. https://doi.org/10.1021/ac100954p
Kertesz V, Cahill JF, Srijanto BR, Collier CP, Vavrek M, Chen B (2020) Absolute quantitation of propranolol from 200-mum regions of mouse brain and liver thin tissues using laser ablation-dropletProbe-mass spectrometry. Rapid Commun Mass Spectrom 35:e9010. https://doi.org/10.1002/rcm.9010
Pan N, Rao W, Kothapalli NR, Liu R, Burgett AW, Yang Z (2014) The single-probe: a miniaturized multifunctional device for single cell mass spectrometry analysis. Anal Chem 86(19):9376–9380. https://doi.org/10.1021/ac5029038
Yin R, Burnum-Johnson KE, Sun X, Dey SK, Laskin J (2019) High spatial resolution imaging of biological tissues using nanospray desorption electrospray ionization mass spectrometry. Nat Protoc 14(12):3445–3470. https://doi.org/10.1038/s41596-019-0237-4
Chen B, Vavrek M, Cancilla MT (2020) Optimization of dropletProbe-mass spectrometry for whole-body tissue distribution analysis of drug-like molecules. J Am Soc Mass Spectrom 31(12):2558–2562. https://doi.org/10.1021/jasms.0c00168
Chen B, Vavrek M, Gundersdorf R, Zhong W, Cancilla MT (2020) Combining MALDI mass spectrometry imaging and droplet-base surface sampling analysis for tissue distribution, metabolite profiling, and relative quantification of cyclic peptide melanotan II. Anal Chim Acta 1125:279–287. https://doi.org/10.1016/j.aca.2020.05.050
Kertesz V, Weiskittel TM, Van Berkel GJ (2015) An enhanced droplet-based liquid microjunction surface sampling system coupled with HPLC-ESI-MS/MS for spatially resolved analysis. Anal Bioanal Chem 407(8):2117–2125. https://doi.org/10.1007/s00216-014-8287-5
Kertesz V, Van Berkel GJ (2014) Sampling reliability, spatial resolution, spatial precision, and extraction efficiency in droplet-based liquid microjunction surface sampling. Rapid Commun Mass Spectrom 28(13):1553–1560. https://doi.org/10.1002/rcm.6931
Acknowledgments
The work of V.K. was supported by the US Department of Energy, Office of Science, Biological and Environmental Research, Bioimaging Science Program. The authors acknowledge Alain Creissen and Sloan Tilley (HTX Technology) and Matt Orcutt (Resolution Labs) for providing technical support of dropletProbe MS. We acknowledge Scott Fauty (Merck & Co., Inc., Kenilworth, NJ, USA) for performing animal experiments.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Chen, B., Vavrek, M., Cancilla, M.T., Kertesz, V. (2022). Development and Application of DropletProbe Mass Spectrometry for Examining Biodistribution of Therapeutics. In: Lee, YJ. (eds) Mass Spectrometry Imaging of Small Molecules. Methods in Molecular Biology, vol 2437. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2030-4_12
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2030-4_12
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2029-8
Online ISBN: 978-1-0716-2030-4
eBook Packages: Springer Protocols