Abstract
This chapter describes the application of immunohistochemistry and in situ mRNA detection to vibratome sections derived from chicken and mouse inner ears. The protocols portray simple strategies to investigate the cellular organization of vestibular and cochlear sensory epithelia. All inner cell types are confidently identified due to the excellent three-dimensional preservation of the inner ear tissue in vibratome sections. Shown are examples for detecting proteins that label cell subtypes as well as subcellular structures such as synapses. Vibratome sections are suitable for conventional colorimetric in situ hybridization as well as fluorescence-based hybridization chain reaction.
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Scheibinger, M., Janesick, A., Diaz, G.H., Heller, S. (2022). Immunohistochemistry and In Situ mRNA Detection Using Inner Ear Vibratome Sections. In: Groves, A.K. (eds) Developmental, Physiological, and Functional Neurobiology of the Inner Ear. Neuromethods, vol 176. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2022-9_3
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DOI: https://doi.org/10.1007/978-1-0716-2022-9_3
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2021-2
Online ISBN: 978-1-0716-2022-9
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