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Cell-Free Gene Expression pp 293–302Cite as

Assembly of RNA Nanostructures from Double-Crossover Tiles

Part of the Methods in Molecular Biology book series (MIMB,volume 2433)

Abstract

Artificial self-assembling RNA scaffolds can be produced from many types of RNA motifs that are rationally designed. These scaffolds are of interest as nanoscale organizers, with applications in drug delivery and synthetic cells. Here we describe design strategies, production methods, and imaging of micrometer-sized RNA nanotubes and lattices that assemble from RNA tiles comprising multiple distinct strands.

Key words

  • RNA
  • RNA assembly
  • RNA nanotubes
  • RNA lattices

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  • DOI: 10.1007/978-1-0716-1998-8_18
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Acknowledgments

Elisa Franco acknowledges support from the National Science Foundation through the CAREER award DMR 1938194. Jaimie Marie Stewart is a Merck Awardee of the Life Sciences Research Foundation.

Author information

Authors and Affiliations

  1. Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA, USA

    Jaimie Marie Stewart

  2. ChromaCode, Inc., Carlsbad, CA, USA

    Hari K. K. Subramanian

  3. Mechanical and Aerospace Engineering, Bioengineering, and Molecular Biology Institute, University of California at Los Angeles, Los Angeles, CA, USA

    Elisa Franco

Authors
  1. Jaimie Marie Stewart
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  2. Hari K. K. Subramanian
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  3. Elisa Franco
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Corresponding author

Correspondence to Elisa Franco .

Editor information

Editors and Affiliations

  1. Department of Chemical and Biological Engineering, Chemistry of Life Processes Institute, and Center for Synthetic Biology, Northwestern University, Evanston, IL, USA

    Ph.D. Ashty S. Karim

  2. Department of Chemical and Biological Engineering, Chemistry of Life Processes Institute, Center for Synthetic Biology, Robert H. Lurie Comprehensive Cancer Center, and Simpson Querrey Institute, Northwestern University, Evanston, IL, USA

    Ph.D. Michael C. Jewett

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Stewart, J.M., Subramanian, H.K.K., Franco, E. (2022). Assembly of RNA Nanostructures from Double-Crossover Tiles. In: Karim, A.S., Jewett, M.C. (eds) Cell-Free Gene Expression. Methods in Molecular Biology, vol 2433. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1998-8_18

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  • DOI: https://doi.org/10.1007/978-1-0716-1998-8_18

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1997-1

  • Online ISBN: 978-1-0716-1998-8

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