Abstract
Intracellular trafficking of organelles driven by molecular motors underlies essential cellular processes. Mitochondria, the powerhouses of the cell, are one of the major cargoes of molecular motors. Efficient distribution of mitochondria ensures cellular fitness while defects in this process contribute to severe pathologies, such as neurodegenerative diseases. Reconstitution of the mitochondrial microtubule-based transport in vitro in a bottom-up approach provides a powerful tool to investigate the mitochondrial trafficking machinery in a controlled environment in the absence of complex intracellular interactions. In this chapter, we describe the procedures for achieving such reconstitution of mitochondrial transport.
Key words
- Molecular motors
- Adaptor proteins
- Kinesin-1
- TRAK
- Mitochondria
- Motility assay
- TIRF microscopy
- Interference reflection microscopy
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Puttrich, V., Rohlena, J., Braun, M., Lansky, Z. (2022). In Vitro Reconstitution of Molecular Motor-Driven Mitochondrial Transport. In: Vagnoni, A. (eds) Axonal Transport. Methods in Molecular Biology, vol 2431. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1990-2_28
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DOI: https://doi.org/10.1007/978-1-0716-1990-2_28
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