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Quantification of Muscle Stem Cell Differentiation Using Live-Cell Imaging and Eccentricity Measures

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Stem Cell Assays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2429))

Abstract

Culturing primary muscle stem cells ex vivo is a useful method for studying this cell population in controlled environments. Primary muscle stem cells respond to external stimuli differently than immortalized myoblasts (C2C12 cells), making ex vivo culture of muscle stem cells an important tool in understanding cell responses to stimuli. Primary muscle stem cells cultured ex vivo retain a majority of the characteristics they possess in vivo such as the abilities to differentiate into multinucleated structures, and self-renew a stem cell-like population. In this chapter, we describe methods for isolating primary muscle stem cells, controlled differentiation into myotubes, and quantification of differentiation using IncuCyte live cell imaging and analysis software.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA

    Paige C. Arneson-Wissink & Jason D. Doles

Authors
  1. Paige C. Arneson-Wissink
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  2. Jason D. Doles
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Corresponding author

Correspondence to Jason D. Doles .

Editor information

Editors and Affiliations

  1. Stem Cell and Cancer Biology Laboratory, Division of Experimental Medicine and Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA

    Nagarajan Kannan

  2. Hull York Medical School, York, UK

    Philip Beer

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Arneson-Wissink, P.C., Doles, J.D. (2022). Quantification of Muscle Stem Cell Differentiation Using Live-Cell Imaging and Eccentricity Measures. In: Kannan, N., Beer, P. (eds) Stem Cell Assays. Methods in Molecular Biology, vol 2429. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1979-7_31

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  • DOI: https://doi.org/10.1007/978-1-0716-1979-7_31

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1978-0

  • Online ISBN: 978-1-0716-1979-7

  • eBook Packages: Springer Protocols

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