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Isolation and Characterization of Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells

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Book cover Stem Cell Assays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2429))

Abstract

The safety and efficacy of mesenchymal stem cells/marrow stromal cells (MSC) have been widely studied. Since they are hypoimmunogenic, MSC can escape immune recognition, thus making them an attractive tool in clinical settings beyond autologous cell-based therapy. Paracrine factors including extracellular vesicles (EVs) released by MSC play a significant role in exerting therapeutic effects of MSC. Since their first discovery, MSC-EVs have been widely studied in an attempt to tackle the mechanisms of their therapeutic effects in various disease models. However, currently there are no standard methods to isolate EVs. Here, we describe a differential centrifugation-based protocol for isolation of EVs derived from human umbilical cord MSC (huc-MSC). In addition, the protocol describes methods for characterization of the EVs using transmission electron microscope, Western blot, and nanoparticle tracking analysis.

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Acknowledgements

This study was supported by the Universiti Sains Malaysia Research University Grant Scheme (1001/CIPPT/8012203). The authors also acknowledged the CryoCord Sdn Bhd (Malaysia) for providing the human umbilical cord-derived mesenchymal stem cells to be used in our study. The authors declare that there is no potential conflict of interest.

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Authors and Affiliations

  1. Lung Stem Cell and Gene Therapy Group, Regenerative Medicine Cluster, Advanced Medical and Dental Institute (IPPT), Universiti Sains Malaysia, Kepala Batas, Penang, Malaysia

    Noridzzaida Ridzuan & Badrul Hisham Yahaya

  2. Stem Cell Biology and Regenerative Medicine Group, School of Pharmacy, University of Reading, Reading, UK

    Darius Widera

Authors
  1. Noridzzaida Ridzuan
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  2. Darius Widera
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  3. Badrul Hisham Yahaya
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Corresponding author

Correspondence to Badrul Hisham Yahaya .

Editor information

Editors and Affiliations

  1. Stem Cell and Cancer Biology Laboratory, Division of Experimental Medicine and Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA

    Nagarajan Kannan

  2. Hull York Medical School, York, UK

    Philip Beer

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Ridzuan, N., Widera, D., Yahaya, B.H. (2022). Isolation and Characterization of Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells. In: Kannan, N., Beer, P. (eds) Stem Cell Assays. Methods in Molecular Biology, vol 2429. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1979-7_18

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  • DOI: https://doi.org/10.1007/978-1-0716-1979-7_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1978-0

  • Online ISBN: 978-1-0716-1979-7

  • eBook Packages: Springer Protocols

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