Abstract
In chicken, Salmonella Enteritidis and Salmonella Typhimurium, the two main serotypes isolated in human infections, can persist in the host organism for many weeks and up to many years without causing any symptoms. This persistence generally occurs after a short systemic infection that may either lead to death of very young birds or develop into cecal asymptomatic persistence, which is often accompanied by a high level of bacterial excretion, facilitating Salmonella transmission to counterparts. Here we describe two models of chick infection. The first model reproduces well the poultry infection in farm flocks. Numerous reinfections and animal-animal recontaminations occur leading to a high level of cecal colonization and fecal excretion in all chicks in the flock, over several weeks. In the second model, these animal reinfections and recontaminations are hampered leading to heterogeneity of infection characterized by the presence of low and super-shedders. This model allows for more mechanistic studies of Salmonella/chicks interactions as animal recontaminations are lowered.
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Acknowledgments
These models of infection were developed with the support of the Institut National de la Recherche Agronomique and with the transnational Emida projects “Healthy gut” and “Difagh” as well as the Aniwha project “AWAP.” “This work also received funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 773830: One Health European Joint Programme, MoMIR-PPC project.” We would like to thank the “Ministry of Agriculture CASDAR,” ITAVI and INRAE for funding the research project CASDAR E-Broilertrack n° 18ART1832 which made it possible to take the photographs of experimental breeding methods. We would like to thank Angélique Travel and Pauline Creach of the ITAVI poultry sector and the CASDAR project managers for the photographs. We also thank the members of the Experimental Infectiology Platform (UE-1277 PFIE, INRAE Centre Val de Loire, Nouzilly, France, https://doi.org/10.15454/1.5535888072272498e12) and particularly Stéphane Abrioux, Mickaël Riou, and Laurence Merat; Sylvain Breton, Alexis Pléau, Arnaud Faurie, Olivier Dubès, and Mylène Girault.
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Velge, P., Menanteau, P., Chaumeil, T., Barilleau, E., Trotereau, J., Virlogeux-Payant, I. (2022). Two In Vivo Models to Study Salmonella Asymptomatic Carrier State in Chicks. In: Gal-Mor, O. (eds) Bacterial Virulence. Methods in Molecular Biology, vol 2427. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1971-1_20
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DOI: https://doi.org/10.1007/978-1-0716-1971-1_20
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