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Mouse Model to Study Salmonella-Induced Colitis

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Bacterial Virulence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2427))

Abstract

Salmonella efficiently colonizes the cecum and proximal colon of mice where it induces inflammation resulting in colitis. To study intestinal infection of non-typhoidal Salmonella enterica serovars in mice, the colonization resistance of the intestine is overcome by transiently reducing the gut microbiota by an oral antibiotic treatment 1 day prior to infection with Salmonella. The in vivo colitis model is crucial for understanding the role of mucosal host defenses, analysis of histopathological changes, and the identification of host and bacterial factors leading to acute infections or facilitating bacterial persistence.

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Acknowledgments

GAG is supported by the Deutsche Forschungsgemeinschaft (DFG) priority program SPP1656/2, the German Federal Ministry of Education and Research (BMBF) Infect-ERA consortium grant 031L0093B and DFG collaborative research center SFB 900 TP08 (Project number 158989968).

Ethics statement: Images in Fig. 3 were obtained in animal experiments that were performed in accordance with the German Animal Protection Law and were approved by the Animal Research Ethics Board of the Ministry of Environment, Kiel, Germany (approval # V312–7224.123-3).

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Authors and Affiliations

  1. Institute of Medical Microbiology and Hospital Epidemiology and German Center for Infection Research (DZIF), Partner Site Hannover-Braunschweig, Hannover Medical School, Hannover, Germany

    Katrin Ehrhardt & Guntram A. Grassl

Authors
  1. Katrin Ehrhardt
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  2. Guntram A. Grassl
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Corresponding author

Correspondence to Guntram A. Grassl .

Editor information

Editors and Affiliations

  1. The Infectious Diseases Research Laboratory, Sheba Medical Center, Tel-Hashomer, Israel

    Ohad Gal-Mor

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Ehrhardt, K., Grassl, G.A. (2022). Mouse Model to Study Salmonella-Induced Colitis. In: Gal-Mor, O. (eds) Bacterial Virulence. Methods in Molecular Biology, vol 2427. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1971-1_17

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  • DOI: https://doi.org/10.1007/978-1-0716-1971-1_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1970-4

  • Online ISBN: 978-1-0716-1971-1

  • eBook Packages: Springer Protocols

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