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A Pipeline for Peptide Detection Using Multiple Decoys

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2426))

Abstract

Target–decoy competition has been commonly used for over a decade to control the false discovery rate when analyzing tandem mass spectrometry (MS/MS) data. We recently developed a framework that uses multiple decoys to increase the number of detected peptides in MS/MS data. Here, we present a pipeline of Apache licensed, open-source software that allows the user to readily take advantage of our framework.

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References

  1. Elias JE, Gygi SP (2007) Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry. Nat. Methods 4(3):207–214. https://doi.org/10.1038/nmeth1019

  2. He K, Fu Y, Zeng WF, Luo L, Chi H, Liu C, Qing LY, Sun RX, He SM (2015) A theoretical foundation of the target-decoy search strategy for false discovery rate control in proteomics. https://arxiv.org/abs/1501.00537v1

  3. Granholm V, Navarro JF, Noble WS, Käll L (2013) Determining the calibration of confidence estimation procedures for unique peptides in shotgun proteomics. J Proteom. 80:123–131. https://doi.org/10.1016/j.jprot.2012.12.007

  4. Emery K, Hasam S, Noble WS, Keich U (2020) Multiple competition-based FDR control and its application to peptide detection. In: Lecture notes in computer science. Springer International Publishing, Cham, pp 54–71. https://doi.org/10.1007/978-3-030-45257-5_4

  5. Keich U, Noble WS (2017) Controlling the FDR in imperfect database matches applied to tandem mass spectrum identification. J Amer Statist Assoc 113:973–982. https://doi.org/10.1080/01621459.2017.1375931

  6. Keich U, Noble WS (2017) Progressive calibration and averaging for tandem mass spectrometry statistical confidence estimation: Why settle for a single decoy. In: Sahinalp S (ed) Proceedings of the international conference on research in computational biology (RECOMB). Springer, Lecture Notes in Computer Science, vol 10229. Springer, Berlin, pp 99–116. https://doi.org/10.1007/978-3-319-56970-3_7

  7. Keich U, Tamura K, Noble WS (2018) Averaging strategy to reduce variability in target-decoy estimates of false discovery rate. J Proteome Res 18(2):585–593. https://doi.org/10.1021/acs.jproteome.8b00802

  8. Park CY, Klammer AA, Käll L, MacCoss MJ, Noble WS (2008) Rapid and accurate peptide identification from tandem mass spectra. J Proteome Res 7(7):3022–3027. https://doi.org/10.1021/pr800127y

  9. Emery K (2019) Multicomp: Multiple Competition FDR Control. R package version 0.2.0

    Google Scholar 

  10. Schittmayer M, Fritz K, Liesinger L, Griss J, Birner-Gruenberger R (2016) Cleaning out the litterbox of proteomic scientists’ favorite pet: optimized data analysis avoiding trypsin artifacts. J Proteome Res 15(4):1222–1229. https://doi.org/10.1021/acs.jproteome.5b01105

  11. Perez-Riverol Y, Csordas A, Bai J, Bernal-Llinares M, Hewapathirana S, Kundu DJ, Inuganti A, Griss J, Mayer G, Eisenacher M, Pérez E, Uszkoreit J, Pfeuffer J, Sachsenberg T, Yılmaz Ş, Tiwary S, Cox J, Audain E, Walzer M, Jarnuczak AF, Ternent T, Brazma A, Vizcaíno JA (2018) The PRIDE database and related tools and resources in 2019: improving support for quantification data. Nucleic Acids Res 47(D1):D442–D450. https://doi.org/10.1093/nar/gky1106

  12. Barber RF, Candès EJ (2015) Controlling the false discovery rate via knockoffs. Ann Statist 43(5):2055–2085. https://doi.org/10.1214/15-AOS1337

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Correspondence to Uri Keich .

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Hasam, S., Emery, K., Noble, W.S., Keich, U. (2023). A Pipeline for Peptide Detection Using Multiple Decoys. In: Burger, T. (eds) Statistical Analysis of Proteomic Data. Methods in Molecular Biology, vol 2426. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1967-4_2

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  • DOI: https://doi.org/10.1007/978-1-0716-1967-4_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1966-7

  • Online ISBN: 978-1-0716-1967-4

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