Abstract
Immunohistochemistry (IHC) is a powerful laboratory technique that is widely used in a variety of settings, including clinical medicine and research. Enzymatic immunohistochemistry refers to the process of detecting cell surface proteins or antigens using enzyme-labeled antibodies. The site and specificity of the antibody binding may be identified using direct labeling techniques or through secondary labeling methods. The antibody-antigen interaction is ultimately visualized using a chromogenic detection process, in which the enzyme conjugated to the antibody cleaves a substrate to produce a stable, colored precipitate.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Cuello AC (1993) Immunohistochemistry II. Wiley Press, New York
Mason TE, Phifer RF, Spicer SS (1969) An immunoglobulin-enzyme bridge method for localizing tissue antigens. J Histochem Cytochem 17:563–569
Van der Loos CM (1999) Immunoenzyme multiple staining methods. BIOS Scientific Ltd., Milton Park
Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Henrich Lobo, R. (2022). Enzymatic Immunohistochemistry. In: Del Valle, L. (eds) Immunohistochemistry and Immunocytochemistry. Methods in Molecular Biology, vol 2422. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1948-3_8
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1948-3_8
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1947-6
Online ISBN: 978-1-0716-1948-3
eBook Packages: Springer Protocols