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Introduction to Immunohistochemistry: From to Evolving Science to Timeless Art

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Immunohistochemistry and Immunocytochemistry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2422))

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Abstract

Immunohistochemistry and all techniques that use antibodies and fluorescence are widespread, essential and irreplaceable tools used in both research laboratory settings and diagnostic pathology laboratories. The field was born approximately 80 years ago, with the idea that antibodies could be tagged with fluorescent substances and used to detect antigens in cells and microorganisms, and has vertiginously evolved since; these advances have come in all aspects of the methodology, tissue fixation, generation of antibodies, monoclonal antibodies, signal amplification, antigen retrieval, signal amplification, microscopy and have become increasingly sophisticated, from in situ hybridization, in situ proximity ligation assay, flow cytometry, comet assay, to multiplexing and green fluorescent protein reconstitution, yielding Nobel Prizes along the way and generating invaluable scientific and diagnostic advances as well as timeless beautiful images.

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Authors and Affiliations

  1. Department of Pathology and Medicine & Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, LA, USA

    Luis Del Valle

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  1. Luis Del Valle
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Correspondence to Luis Del Valle .

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  1. Department of Pathology and Medicine & Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, LA, USA

    Luis Del Valle

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Del Valle, L. (2022). Introduction to Immunohistochemistry: From to Evolving Science to Timeless Art. In: Del Valle, L. (eds) Immunohistochemistry and Immunocytochemistry. Methods in Molecular Biology, vol 2422. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1948-3_1

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  • DOI: https://doi.org/10.1007/978-1-0716-1948-3_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1947-6

  • Online ISBN: 978-1-0716-1948-3

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