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Estrogen Receptors pp 95–112Cite as

In Situ Hybridization of Estrogen Receptors α and β in the Human Testis

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Part of the Methods in Molecular Biology book series (MIMB,volume 2418)

Abstract

In situ hybridization (ISH) is an excellent method for detecting RNA in histological sections, both to detect gene expression and to assign gene expression to a distinct cell population. Therefore, ISH may be used in basic cell biology to detect the expression of certain genes within a tissue containing various cell populations. Here, we describe the detection and cellular localization of two estrogen receptors, both isoforms of the genomic estrogen receptor (ERα and ERβ) in the human testis.

Key words

  • In situ hybridization
  • Testis
  • Estrogen receptors
  • Molecular biology
  • Gene expression
  • Cellular localization

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  • DOI: 10.1007/978-1-0716-1920-9_7
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Authors and Affiliations

  1. Institute for Veterinary Anatomy, Histology and Embryology, Justus Liebig University, Giessen, Germany

    Katja Hartmann & Daniela Fietz

Authors
  1. Katja Hartmann
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  2. Daniela Fietz
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Corresponding author

Correspondence to Daniela Fietz .

Editor information

Editors and Affiliations

  1. Sanford School of Medicine, University of South Dakota, Vermillion, SD, USA

    Kathleen M. Eyster

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Hartmann, K., Fietz, D. (2022). In Situ Hybridization of Estrogen Receptors α and β in the Human Testis. In: Eyster, K.M. (eds) Estrogen Receptors. Methods in Molecular Biology, vol 2418. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1920-9_7

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  • DOI: https://doi.org/10.1007/978-1-0716-1920-9_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1919-3

  • Online ISBN: 978-1-0716-1920-9

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