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In Situ Hybridization of Estrogen Receptors α and β in the Human Testis

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Part of the Methods in Molecular Biology book series (MIMB,volume 2418)

Abstract

In situ hybridization (ISH) is an excellent method for detecting RNA in histological sections, both to detect gene expression and to assign gene expression to a distinct cell population. Therefore, ISH may be used in basic cell biology to detect the expression of certain genes within a tissue containing various cell populations. Here, we describe the detection and cellular localization of two estrogen receptors, both isoforms of the genomic estrogen receptor (ERα and ERβ) in the human testis.

Key words

  • In situ hybridization
  • Testis
  • Estrogen receptors
  • Molecular biology
  • Gene expression
  • Cellular localization

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  • DOI: 10.1007/978-1-0716-1920-9_7
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Correspondence to Daniela Fietz .

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Hartmann, K., Fietz, D. (2022). In Situ Hybridization of Estrogen Receptors α and β in the Human Testis. In: Eyster, K.M. (eds) Estrogen Receptors. Methods in Molecular Biology, vol 2418. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1920-9_7

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  • DOI: https://doi.org/10.1007/978-1-0716-1920-9_7

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1919-3

  • Online ISBN: 978-1-0716-1920-9

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