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Synchronizing Mammalian Cells for Mitotic Analysis of the Localization of Survivin

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Mitosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2415))

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Abstract

This paper describes an easy method to enrich the harvest of adherent mammalian cells at each stage of mitosis (from prometaphase to cytokinesis) by combining Eg5 inhibition using dimethylenastron (DMA) with mitotic shake-off, followed by timed release from the drug.

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Authors and Affiliations

  1. School of Life Sciences, University of Nottingham, Medical School, Queen’s Medical Centre, Nottingham, UK

    Sally P. Wheatley

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  1. Sally P. Wheatley
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Corresponding author

Correspondence to Sally P. Wheatley .

Editor information

Editors and Affiliations

  1. The Hormel Institute, University of Minnesota, Austin, MN, USA

    Edward H. Hinchcliffe

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Wheatley, S.P. (2022). Synchronizing Mammalian Cells for Mitotic Analysis of the Localization of Survivin. In: Hinchcliffe, E.H. (eds) Mitosis. Methods in Molecular Biology, vol 2415. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1904-9_7

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  • DOI: https://doi.org/10.1007/978-1-0716-1904-9_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1903-2

  • Online ISBN: 978-1-0716-1904-9

  • eBook Packages: Springer Protocols

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