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Imaging Chambers for Arabidopsis Seedlings for Mitotic Studies

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Mitosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2415))

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Abstract

Flowering plants evolved away from creating centrosomes or conventional microtubule organizing centers. Therein, plants have posed a long-standing challenge to many of the conventional ideas for mitotic spindle construction and the process of chromosome segregation. The Arabidopsis seedling has emerged as a leading model for plant cell biological studies of the cytoskeleton and vesicle trafficking. Here we describe methods for creating a reusable chamber for mitotic studies in both seedling root and shoot cells with instruction for best practices with conventional microscopic techniques.

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Authors and Affiliations

  1. Department of Biology, Indiana University, Bloomington, IN, USA

    Sidney L. Shaw, Mathew Siebe & Timothy Cioffi

Authors
  1. Sidney L. Shaw
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  2. Mathew Siebe
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  3. Timothy Cioffi
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Corresponding author

Correspondence to Sidney L. Shaw .

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Editors and Affiliations

  1. The Hormel Institute, University of Minnesota, Austin, MN, USA

    Edward H. Hinchcliffe

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Shaw, S.L., Siebe, M., Cioffi, T. (2022). Imaging Chambers for Arabidopsis Seedlings for Mitotic Studies. In: Hinchcliffe, E.H. (eds) Mitosis. Methods in Molecular Biology, vol 2415. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1904-9_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1904-9_4

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1903-2

  • Online ISBN: 978-1-0716-1904-9

  • eBook Packages: Springer Protocols

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