Abstract
Extracellular membrane vesicles (EMVs) produced by Gram-negative bacteria are useful as a vaccine platform. During growth in broth at 18 °C, Shewanella vesiculosa HM13 produces a large number of EMVs that contain a 49-kDa major cargo protein, named P49. Enhanced green fluorescent protein fused to the C-terminus of P49 is delivered to EMVs, suggesting that P49 is useful as a carrier to target foreign proteins to EMVs for production of artificial EMVs with desired functions. This method is potentially useful for the preparation of designed vaccines and is described in detail in this chapter.
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Acknowledgments
This work was supported in part by JSPS KAKENHI (JP17H04598, JP18K19178, and JP20K20570 to TK and JP16K14885 and JP20K05786 to JK) and a grant from the Institute for Fermentation, Osaka (L-2019-2-012 to TK). TEM observations were performed in collaboration with the Analysis and Development System for Advanced Materials (ADAM) at the Research Institute for Sustainable Humanosphere, Kyoto University.
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Kawamoto, J., Kurihara, T. (2022). Membrane Vesicles Produced by Shewanella vesiculosa HM13 as a Prospective Platform for Secretory Production of Heterologous Proteins at Low Temperatures. In: Bidmos, F., Bossé, J., Langford, P. (eds) Bacterial Vaccines. Methods in Molecular Biology, vol 2414. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1900-1_12
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DOI: https://doi.org/10.1007/978-1-0716-1900-1_12
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