Abstract
Experimental increase of cytosine-phosphate-guanine (CpG) dinucleotides in an RNA virus genome impairs infection. Beneficially, this weak infection may lead to robust antiviral host immunity providing a cutting-edge approach for vaccines. For example, we have recently demonstrated that recoded Zika virus variants with the increased CpG content showed considerable attenuated infection phenotypes and protection against lethal challenge in mice. Here, we describe the workflow for the design and generation of CpG-recoded Zika virus vaccine candidates. The workflow can be adapted for other viruses.
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Published as VIDO-InterVac manuscript series no. 912.
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Trus, I., Udenze, D., Karniychuk, U. (2022). Generation of CpG-Recoded Zika Virus Vaccine Candidates. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 2410. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1884-4_14
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DOI: https://doi.org/10.1007/978-1-0716-1884-4_14
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