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Recent Advances in Plant Gene Silencing Methods

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Plant Gene Silencing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2408))

Abstract

With the increasing understanding of fundamentals of gene silencing pathways in plants, various tools and techniques for downregulating the expression of a target gene have been developed across multiple plant species. This chapter provides an insight into the molecular mechanisms of gene silencing and highlights the advancements in various gene silencing approaches. The prominent aspects of different gene silencing methods, their advantages and disadvantages have been discussed. A succinct discussion on the newly emerged microRNA-based technologies like microRNA-induced gene silencing (MIGS) and microRNA-mediated virus-induced gene silencing (MIR-VIGS) are also presented. We have also discussed the gene-editing system like CRISPR-Cas. The prominent bottlenecks in gene silencing methods are the off-target effects and lack of universal applicability. However, the tremendous growth in understanding of this field reflects the potentials for improvements in the currently available approaches and the development of new widely applicable methods for easy, fast, and efficient functional characterization of plant genes.

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Authors and Affiliations

  1. National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India

    Prachi Pandey & Muthappa Senthil-Kumar

  2. Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, OK, USA

    Kirankumar S. Mysore

Authors
  1. Prachi Pandey
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  2. Kirankumar S. Mysore
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  3. Muthappa Senthil-Kumar
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Correspondence to Muthappa Senthil-Kumar .

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Editors and Affiliations

  1. Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, OK, USA

    Kirankumar S. Mysore

  2. National Institute of Plant Genome Research, New Delhi, India

    Muthappa Senthil-Kumar

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Pandey, P., Mysore, K.S., Senthil-Kumar, M. (2022). Recent Advances in Plant Gene Silencing Methods. In: Mysore, K.S., Senthil-Kumar, M. (eds) Plant Gene Silencing. Methods in Molecular Biology, vol 2408. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1875-2_1

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  • DOI: https://doi.org/10.1007/978-1-0716-1875-2_1

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  • Publisher Name: Humana, New York, NY

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