Abstract
Monocytes/macrophages play critical roles in HIV transmission, viral spread (early in infection), and as a reservoir of virus throughout infection. In the current research area in HIV, there has been a recent resurgence of interest in the biology of monocyte subsets and macrophages and their role in HIV pathogenesis, and as long-lived HIV reservoir. Thus, sensitive and specific techniques are needed to measure the impact of these cells in the establishment of the “hard-core” reservoir, and in their capacity to cause a low-level virus production during cART. Here, a protocol is presented for cell culture and HIV-1 infection of granulocyte–macrophage colony-stimulating factor (GM-CSF) differentiated human monocyte-derived macrophages.
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Alteri, C., Piermatteo, L., Silberstein, F.C., Svicher, V., Perno, C.F. (2022). Human Monocyte-Derived Macrophages (MDM): Model 1 (GM-CSF). In: Poli, G., Vicenzi, E., Romerio, F. (eds) HIV Reservoirs. Methods in Molecular Biology, vol 2407. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1871-4_8
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DOI: https://doi.org/10.1007/978-1-0716-1871-4_8
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1870-7
Online ISBN: 978-1-0716-1871-4
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