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The Cultured TCM Model of HIV Latency

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HIV Reservoirs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2407))

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Abstract

Models to study HIV latency have improved our understanding of the mechanisms involved in this process and have helped in the discovery and development of therapeutic strategies to eradicate HIV. Primary cell models are based on the in vitro generation of latently infected cells using CD4T cells isolated from blood, lymph nodes or other lymphoid organs. In this chapter, we describe the generation of HIV latently infected memory CD4T cells using blood naïve CD4T cells from peripheral blood with a phenotype resembling that of central memory CD4T cells. This model can be used to investigate the mechanisms involved in latency as well to develop strategies to target it.

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Acknowledgments

I would like to thank Indra Sarabia and Amanda B. Macedo for their input in the writing and proofreading of the manuscript. The work in A.B.’s lab is currently supported by the National Institute of Allergy and Infectious Diseases and National Institute of Health grants R01-AI124722, R21/R33-AI116212, R01-AI147845, UM1-AI126617, and P30-AI117970.

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Authors and Affiliations

  1. Department of Microbiology, Immunology and Tropical Medicine, George Washington University, Washington, DC, USA

    Alberto Bosque

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  1. Alberto Bosque
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Correspondence to Alberto Bosque .

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Editors and Affiliations

  1. School of Medicine and Human Immuno-Virology (H.I.V.) Group, San Raffaele University and Scientific Institute, Milano, Italy

    Guido Poli

  2. Viral Pathogenesis and Biosafety Group, San Raffaele Scientific Institute, Milano, Italy

    Elisa Vicenzi

  3. Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Fabio Romerio

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Bosque, A. (2022). The Cultured TCM Model of HIV Latency. In: Poli, G., Vicenzi, E., Romerio, F. (eds) HIV Reservoirs. Methods in Molecular Biology, vol 2407. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1871-4_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1871-4_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1870-7

  • Online ISBN: 978-1-0716-1871-4

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