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Analysis of mRNA Translation by Polysome Profiling

Part of the Methods in Molecular Biology book series (MIMB,volume 2404)

Abstract

mRNA translation is a key step in gene expression that allows the cell to qualitatively and quantitatively modulate the cell’s proteome according to intra- or extracellular signals. Polysome profiling is the most comprehensive technique to study both the translation state of mRNAs and the protein machinery associated with the mRNAs being translated. Here we describe the procedure commonly used in our laboratory to gain insights into the molecular mechanisms underlying translation regulation under pathophysiological conditions.

Key words

  • mRNA translation
  • Polysome profiling
  • Translatome
  • Ribosome

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  • DOI: 10.1007/978-1-0716-1851-6_4
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Correspondence to Stefania Millevoi .

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Cammas, A., Herviou, P., Dumas, L., Millevoi, S. (2022). Analysis of mRNA Translation by Polysome Profiling. In: Dassi, E. (eds) Post-Transcriptional Gene Regulation. Methods in Molecular Biology, vol 2404. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1851-6_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1851-6_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1850-9

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