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Enzyme Engineering pp 263–276Cite as

Advanced Enzyme Immobilization Technologies: An Eco-friendly Support, a Polymer-Stabilizing Immobilization Strategy, and an Improved Cofactor Co-immobilization Technique

Part of the Methods in Molecular Biology book series (MIMB,volume 2397)

Abstract

There is a wide variety of protocols for enzyme immobilization, allowing for the reuse of the enzyme, integration in flow bioreactors, and easy separation from the final product. However, none of them have reached a generalized implementation and new immobilization technologies are continuously being developed to improve the properties of the immobilized biocatalysts. In this chapter, we describe three advanced strategies looking at the key points of enzyme immobilization: the sustainability of the support, the recovered activity of the immobilized enzyme, and the reuse of the cofactors. Lignin is presented as a suitable and versatile support for enzyme immobilization, offering a more cost-effective and biodegradable strategy. A cationic polymer is used during the enzyme immobilization procedure to prevent the subunit dissociation of multimeric enzymes as well as to avoid excessive rigidification of the covalently immobilized enzyme. Finally, the reversible co-immobilization of cofactors has been improved by increasing the reactive groups of the support.

Key words

  • Enzyme immobilization
  • Enzyme stability
  • Lignin
  • Polyethyleneimine
  • Cofactor co-immobilization

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Acknowledgments

The authors acknowledge the University of Bern for the “Seal of Excellence Fund” (SELF) postdoctoral grant to support A.I.B.M.

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Authors and Affiliations

  1. Department of Chemistry, Biochemistry and Pharmaceutical Sciences, University of Bern, Bern, Switzerland

    Ana I. Benítez-Mateos & Francesca Paradisi

Authors
  1. Ana I. Benítez-Mateos
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  2. Francesca Paradisi
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Corresponding authors

Correspondence to Ana I. Benítez-Mateos or Francesca Paradisi .

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Editors and Affiliations

  1. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Francesca Magnani

  2. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Chiara Marabelli

  3. Department of Chemistry & Biochemistry, University of Bern, Bern, Switzerland

    Prof. Francesca Paradisi

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Benítez-Mateos, A.I., Paradisi, F. (2022). Advanced Enzyme Immobilization Technologies: An Eco-friendly Support, a Polymer-Stabilizing Immobilization Strategy, and an Improved Cofactor Co-immobilization Technique. In: Magnani, F., Marabelli, C., Paradisi, F. (eds) Enzyme Engineering. Methods in Molecular Biology, vol 2397. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1826-4_14

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  • DOI: https://doi.org/10.1007/978-1-0716-1826-4_14

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