Abstract
A novel approach to address the clinical issue of cell response to wear and corrosion debris from metal orthopedic implants consists of combining cell culturing with wear and corrosion debris generation. A biotribometer equipped with a three-electrode electrochemical chamber operates inside a CO2 incubator. Cells are cultured at the bottom of the chamber. A ceramic ball (hip implant head) is pressed against a metal disc under a constant load, and set in reciprocating rotation. An anodic electrochemical potential can be applied to a metal disc for accelerated corrosion conditions, or the free potential may be monitored.
Measurements of gravimetric and volumetric material loss of the metal disc postwear provide quantitative information that can be put in relation to biological assays (e.g., cell viability and secretion of proinflammatory cytokines). This approach allows for the comparison of candidate metals potentially undergoing tribocorrosion in clinical use. The approach allows to identify the effect of any metastable debris, likely active in vivo.
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Acknowledgments
The authors would like to thank Kathrin Ebinger, Michel P. Laurent, Robin Pourzal, Stephanie McCarthy, Spencer Fullam, Jade He, Nadim J. Hallab, Lauryn A. Samelko, Kyron McAllister (Rush University Medical Center); Joel F. Rodriguez, Thomas A. Bruzan, Matthew J. Schuck (UIC scientific instrument shop); and Prof. Mathew T. Mathew (University of Illinois, College of Medicine at Rockford). This work was supported by the National Institutes of Health (NIH/NIBIB grant number R21EB024039).
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Radice, S., Wimmer, M.A. (2022). Biotribometer for Assessment of Cell and Tissue Toxicity of Orthopedic Metal Implant Debris. In: Rasooly, A., Baker, H., Ossandon, M.R. (eds) Biomedical Engineering Technologies. Methods in Molecular Biology, vol 2394. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1811-0_37
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DOI: https://doi.org/10.1007/978-1-0716-1811-0_37
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