Abstract
Here we describe methods for synthesizing cationic multiarm Avidin (mAv) nanoconstruct that has a wide range of applications in drug delivery and imaging for a variety of negatively charged tissues. The multiarm structure provides multiple sites for covalent conjugation of drugs. We use avidin–biotin reaction that gives the flexibility for conjugating any desired biotinylated drug to mAv by simple mixing at room temperature. We also describe methods to control hydrolysis rates of ester linkers to enable sustained (and tunable) drug release rates in therapeutic doses.
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Acknowledgments
This work was supported by the US Department of Defense through the Congressionally Directed Medical Research Programs (CDMRP) [W81XWH-17-1-0085] and National Institute of Health (R03 EB025903; Trailblazer R21 EB028385).
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He, T., Zhang, C., Bajpayee, A.G. (2022). Charge-Based Multiarm Avidin Nanoconstruct as a Platform Technology for Applications in Drug Delivery. In: Rasooly, A., Baker, H., Ossandon, M.R. (eds) Biomedical Engineering Technologies. Methods in Molecular Biology, vol 2394. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1811-0_28
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DOI: https://doi.org/10.1007/978-1-0716-1811-0_28
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