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Biomedical Engineering Technologies pp 343–376Cite as

Self-Induced Back-Action Actuated Nanopore Electrophoresis (SANE) Sensor for Label-Free Detection of Cancer Immunotherapy-Relevant Antibody-Ligand Interactions

Part of the Methods in Molecular Biology book series (MIMB,volume 2394)

Abstract

We fabricated a novel single molecule nanosensor by integrating a solid-state nanopore and a double nanohole nanoaperture. The nanosensor employs Self-Induced Back-Action (SIBA) for optical trapping and enables SIBA-Actuated Nanopore Electrophoresis (SANE) for concurrent acquisition of bimodal optical and electrical signatures of molecular interactions. This work describes how to fabricate and use the SANE sensor to quantify antibody-ligand interactions. We describe how to analyze the bimodal optical-electrical data to improve upon the discrimination of antibody and ligand versus bound complex compared to electrical measurements alone. Example results for specific interaction detection are described for T-cell receptor-like antibodies (TCRmAbs) engineered to target peptide-presenting Major Histocompatibility Complex (pMHC) ligands, representing a model of target ligands presented on the surface of cancer cells. We also describe how to analyze the bimodal optical-electrical data to discriminate between specific and non-specific interactions between antibodies and ligands. Example results for non-specific interactions are shown for cancer-irrelevant TCRmAbs targeting the same pMHCs, as a control. These example results demonstrate the utility of the SANE sensor as a potential screening tool for ligand targets in cancer immunotherapy, though we believe that its potential uses are much broader.

Key words

  • Solid-state nanopores
  • Dual nanoholes
  • Nanopore translocations
  • Plasmonic optical trapping
  • Dual modality nanosensing
  • Antibody-ligand interactions
  • TCR-like monoclonal antibodies
  • Peptide major histocompatibility complexes (pMHCs)

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  • DOI: 10.1007/978-1-0716-1811-0_20
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Author information

Authors and Affiliations

  1. Department of Electrical Engineering, University of Texas at Arlington, Arlington, TX, USA

    Sai Santosh Sasank Peri & Muhammad Usman Raza

  2. Department of Biology, University of Texas at Arlington, Arlington, TX, USA

    Manoj K. Sabnani & Soroush Ghaffari

  3. Resonant Sensors Incorporated (RSI), Arlington, TX, USA

    Susanne Gimlin & Debra D. Wawro

  4. Department of Mechanical Engineering, Southern Methodist University, Dallas, TX, USA

    Jung Soo Lee & Min Jun Kim

  5. Department of Bioengineering, University of Texas at Arlington, Arlington, TX, USA

    George Alexandrakis

  6. Department of Kinesiology, University of Texas at Arlington, Arlington, TX, USA

    Jon Weidanz

Authors
  1. Sai Santosh Sasank Peri
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  2. Muhammad Usman Raza
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  3. Manoj K. Sabnani
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  4. Soroush Ghaffari
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  6. Debra D. Wawro
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  7. Jung Soo Lee
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  8. Min Jun Kim
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  9. Jon Weidanz
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  10. George Alexandrakis
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Corresponding author

Correspondence to George Alexandrakis .

Editor information

Editors and Affiliations

  1. Division of Cancer Treatment and Diagnosis, National Cancer Institute, Rockville, MD, USA

    Avraham Rasooly

  2. Division of Cancer Treatment and Diagnosis, National Cancer Institute, Rockville, MD, USA

    Houston Baker

  3. Division of Cancer Treatment and Diagnosis, National Cancer Institute, NIH, Bethesda, MD, USA

    Ph.D. Miguel R. Ossandon

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Peri, S.S.S. et al. (2022). Self-Induced Back-Action Actuated Nanopore Electrophoresis (SANE) Sensor for Label-Free Detection of Cancer Immunotherapy-Relevant Antibody-Ligand Interactions. In: Rasooly, A., Baker, H., Ossandon, M.R. (eds) Biomedical Engineering Technologies. Methods in Molecular Biology, vol 2394. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1811-0_20

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  • DOI: https://doi.org/10.1007/978-1-0716-1811-0_20

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