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An Ultracompact Real-Time Fluorescence Loop-Mediated Isothermal Amplification (LAMP) Analyzer

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Biomedical Engineering Technologies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2393))

Abstract

Low-cost access to the highly sensitive and specific detection of the pathogen in the field is a crucial attribute for the next generation point-of-care (POC) platforms. In this work, we developed a real-time fluorescence nucleic acid testing device with automated and scalable sample preparation capability for field malaria diagnosis. The palm-sized battery-powered analyzer equipped with a disposable microfluidic reagent compact disc described in the companion Chap. 16 which facilitates four isothermal nucleic acid tests in parallel from raw blood samples to answer. The platform has a user-friendly interface such as touchscreen LCD and smartphone data connectivity for on-site and remote healthcare delivery, respectively. The chapter mainly focuses on describing integration procedures of the real-time fluorescence LAMP analyzer and the validation of its subsystems. The device cost is significantly reduced compared to the commercial benchtop real-time machine and other existing POC platforms. As a platform technology, self-sustainable, portable, low-cost, and easy-to-use analyzer design should create a new paradigm of molecular diagnosis toward a variety of infectious diseases at the point of need.

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Acknowledgments

This work was supported by grants National Science Foundation under Grant No. 1710831, 1912410, and 1902503. We express our gratitude to Dr. Liwang Cui, Dr. Jun Miao, and Xiaolian Li for providing cultured malaria samples. W.G. acknowledges the support from Penn State Startup Fund.

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Authors and Affiliations

  1. Department of Electrical Engineering, Pennsylvania State University, University Park, PA, USA

    Gihoon Choi & Weihua Guan

  2. Department of Biomedical Engineering, Pennsylvania State University, University Park, PA, USA

    Weihua Guan

Authors
  1. Gihoon Choi
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  2. Weihua Guan
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Corresponding author

Correspondence to Weihua Guan .

Editor information

Editors and Affiliations

  1. Division of Cancer Treatment and Diagnosis, National Cancer Institute, NIH, Bethesda, MD, USA

    Miguel R. Ossandon

  2. Division of Cancer Treatment and Diagnosis, National Cancer Institute, Rockville, MD, USA

    Houston Baker

  3. Division of Cancer Treatment and Diagnosis, National Cancer Institute, Rockville, MD, USA

    Avraham Rasooly

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Choi, G., Guan, W. (2022). An Ultracompact Real-Time Fluorescence Loop-Mediated Isothermal Amplification (LAMP) Analyzer. In: Ossandon, M.R., Baker, H., Rasooly, A. (eds) Biomedical Engineering Technologies. Methods in Molecular Biology, vol 2393. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1803-5_14

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  • DOI: https://doi.org/10.1007/978-1-0716-1803-5_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1802-8

  • Online ISBN: 978-1-0716-1803-5

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