Abstract
Plasmids transmissible by conjugation are responsible for disseminating antibiotic-resistance genes, making plasmid detection relevant for pathogen tracking. We describe the use of a multiplex PCR method for the experimental identification of specific plasmid taxonomic units (PTUs) of transmissible plasmids. The PCR primers were designed to target conserved segments of the relaxase MOB gene of PTUs encoding adaptive traits for enterobacteria (antimicrobial resistance, virulence, and metabolism). In this way, PlasTax-PCR detects the presence of these plasmids and allows their direct assignation to a PTU.
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Acknowledgments
This work was financed by the Spanish Ministry of Science and Innovation (PID2020-117923GB-I00) to FdlC and MPG-B, and by the European Commission, the Joint Programming Initiative in Antimicrobial Resistance and the Ministry of Science and Innovation (UE/STARCS AC16/00039, UE/ST131TS AC16/00043, and PI18/01942) to TMC.
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Cuartas, R., Coque, T.M., de la Cruz, F., Garcillán-Barcia, M.P. (2022). PLASmid TAXonomic PCR (PlasTax-PCR), a Multiplex Relaxase MOB Typing to Assort Plasmids into Taxonomic Units. In: Basu, C. (eds) PCR Primer Design. Methods in Molecular Biology, vol 2392. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1799-1_10
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DOI: https://doi.org/10.1007/978-1-0716-1799-1_10
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