Abstract
The appearance of colony growth sectors on solid medium plates has been described in many fungi. Although the molecular bases of this phenomenon remain largely unknown, possible relationships with genetic or epigenetic changes have been reported. Here we present a method to quantify the frequency of colony growth sectors in Fusarium oxysporum, which can be used to compare different fungal strains and to infer their genetic instability.
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Acknowledgments
The authors acknowledge Esther Martinez Aguilera for valuable technical help. Figures were prepared using the free Biorender online tool. Research in the Di Pietro lab is supported grants BIO2016-78923-R and PID2019-108045RB-I00 from the Spanish Ministerio de Ciencia e Innovación (MICINN). L.G.G is supported by a FPU PhD fellowship from the Spanish Ministerio de Educacion y Ciencia (MEC).
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Díaz, C.L., Gómez-Gil, L., Pérez-Nadales, E., Velasco, G.N., Di Pietro, A. (2022). Quantification and Isolation of Spontaneous Colony Growth Variants. In: Coleman, J. (eds) Fusarium wilt. Methods in Molecular Biology, vol 2391. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1795-3_5
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DOI: https://doi.org/10.1007/978-1-0716-1795-3_5
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