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Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord

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Neural Progenitor Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2389))

Abstract

Whereas neural stem cells and their niches have been extensively studied in the brain, little is known on these cells, their environment, and their function in the adult spinal cord. Adult spinal cord neural stem cells are located in a complex niche surrounding the central canal, and these cells expressed genes which are specifically expressed in the caudal central nervous system (CNS). In-depth characterization of these cells in vivo and in vitro will provide interesting clues on the possibility to utilize this endogenous cell pool to treat spinal cord damages. We describe here a procedure to derive and culture neural spinal cord stem cells from adult mice using the neurosphere method.

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Acknowledgements

Our team is supported by grants from IRP (Switzerland), IRME (France), AFM (France), ANR EU ERANET Neuroniche.

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Authors and Affiliations

  1. Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM , Montpellier, France

    Jean-Philippe Hugnot

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  1. Jean-Philippe Hugnot
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Correspondence to Jean-Philippe Hugnot .

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Editors and Affiliations

  1. Department of Neurosurgery, University of Florida, Gainesville, FL, USA

    Loic P. Deleyrolle

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Hugnot, JP. (2022). Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord. In: Deleyrolle, L.P. (eds) Neural Progenitor Cells. Methods in Molecular Biology, vol 2389. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1783-0_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1783-0_4

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1782-3

  • Online ISBN: 978-1-0716-1783-0

  • eBook Packages: Springer Protocols

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