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Isolation and Culture of Neural Stem/Progenitor Cells from the Postnatal Periventricular Region

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Neural Progenitor Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2389))

Abstract

Due to the complexity of the neural stem cell (NSC) niche organization, the lack of specific NSC markers, and the difficulty of long-term tracking these cells and their progeny in vivo, the functional properties of the endogenous NSCs remain largely unexplored. These limitations have led to the development of methodologies to efficiently isolate, expand, and differentiate NSCs ex vivo. We describe here the peculiarities of the neurosphere assay (NSA) as a methodology that allows to efficiently isolate, expand, and differentiate somatic NSCs derived from the postnatal and adult forebrain periventricular region while preserving proliferation, self-renewal, and multipotency, the main attributes that provide their functional identification.

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Authors and Affiliations

  1. San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, Milan, Italy

    Alessandra Ricca, Federica Cascino & Angela Gritti

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  1. Alessandra Ricca
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  2. Federica Cascino
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  3. Angela Gritti
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Correspondence to Angela Gritti .

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Editors and Affiliations

  1. Department of Neurosurgery, University of Florida, Gainesville, FL, USA

    Loic P. Deleyrolle

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Ricca, A., Cascino, F., Gritti, A. (2022). Isolation and Culture of Neural Stem/Progenitor Cells from the Postnatal Periventricular Region. In: Deleyrolle, L.P. (eds) Neural Progenitor Cells. Methods in Molecular Biology, vol 2389. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1783-0_2

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  • DOI: https://doi.org/10.1007/978-1-0716-1783-0_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1782-3

  • Online ISBN: 978-1-0716-1783-0

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