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Generating Cerebral Organoids from Human Pluripotent Stem Cells

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Neural Progenitor Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2389))

Abstract

3D brain organoids derived from human pluripotent stem cells (hPSCs) possess the remarkable ability to self-organize and differentiate into tissue resembling the early human fetal brain. Brain organoids provide a powerful tool for studying human brain development and disease in an in vitro system. Here we describe a protocol for the differentiation of hPSCs to human cerebral organoids using a commercially available kit (STEMdiff™ Cerebral Organoid Kit) and discuss methods to scale up the protocol in a high-throughput manner.

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Acknowledgments

We’d like to thank Marianne Chomiak for her feedback on the manuscript.

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Author notes

  1. Leon Chew, Adam Añonuevo contributed equally to this work.

Authors and Affiliations

  1. STEMCELL Technologies, Vancouver, BC, Canada

    Leon Chew, Adam Añonuevo & Erin Knock

Authors
  1. Leon Chew
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  2. Adam Añonuevo
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  3. Erin Knock
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Corresponding author

Correspondence to Erin Knock .

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Editors and Affiliations

  1. Department of Neurosurgery, University of Florida, Gainesville, FL, USA

    Loic P. Deleyrolle

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Chew, L., Añonuevo, A., Knock, E. (2022). Generating Cerebral Organoids from Human Pluripotent Stem Cells. In: Deleyrolle, L.P. (eds) Neural Progenitor Cells. Methods in Molecular Biology, vol 2389. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1783-0_15

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  • DOI: https://doi.org/10.1007/978-1-0716-1783-0_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1782-3

  • Online ISBN: 978-1-0716-1783-0

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