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Invariant Natural Killer T-Cells pp 59–69Cite as

Isolation and Detection of Murine iNKT Cells in Different Organs

Part of the Methods in Molecular Biology book series (MIMB,volume 2388)

Abstract

The invariant NKT (iNKT) cells are innate-like lymphocytes that share phenotypic and functional characteristics with NK cells and T cells, playing an important role in both human and mouse physiology and disease and bridging the gap between the innate and adaptive immune responses. The frequency and subtypes of iNKT cells in major immune organs are different, which also determines the regional immune characteristics of iNKT cells. Here, we report a protocol about the isolation of iNKT cells in the thymus, spleen, and liver of C57BL/6, CD1d−/−, and Jα18−/− mice.

Key words

  • iNKT cells
  • Spleen
  • Thymus
  • Liver

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  • DOI: 10.1007/978-1-0716-1775-5_5
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Author notes

  1. Mengqing Cong and Xiang Li contributed equally to this work.

Authors and Affiliations

  1. Division of Life Sciences and Medicine, Department of Medical Oncology, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, China

    Mengqing Cong, Haopeng Fang, Xucai Zheng & Bofeng Li

  2. Hefei National Laboratory for Physical Sciences at Microscale, Laboratory of Structural Immunology, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China

    Mengqing Cong, Xiang Li, Haopeng Fang, Li Bai & Bofeng Li

Authors
  1. Mengqing Cong
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  2. Xiang Li
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  3. Haopeng Fang
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  4. Li Bai
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  5. Xucai Zheng
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  6. Bofeng Li
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Corresponding author

Correspondence to Bofeng Li .

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Editors and Affiliations

  1. Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

    Ph.D. Chaohong Liu

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Cong, M., Li, X., Fang, H., Bai, L., Zheng, X., Li, B. (2021). Isolation and Detection of Murine iNKT Cells in Different Organs. In: Liu, C. (eds) Invariant Natural Killer T-Cells. Methods in Molecular Biology, vol 2388. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1775-5_5

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  • DOI: https://doi.org/10.1007/978-1-0716-1775-5_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1774-8

  • Online ISBN: 978-1-0716-1775-5

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