Abstract
A comprehensive study of the cellular components of the immune system demands both deep and broad immunophenotyping of numerous cell subsets in an effective and practical way. Novel full-spectrum technology reveals the complete emission spectrum of each dye maximizing the amount of information that can be obtained on a single sample regarding conventional flow cytometry and provide an expanded knowledge of biological processes. In this chapter, we describe a 37-color protocol that allows to identify more than 45 different cell populations on whole blood samples of SARS-CoV-2-infected patients.
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09 March 2022
The chapter was inadvertently published with incorrect figure legends, reference citations, and order of references.
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Acknowledgments
This work was partially supported by Instituto de Salud Carlos III (Ministry of Health, Spain) grants COV20_00388, COV20_00416, and COV20_00654. Cytek Aurora Spectral Cytometer was funded by Spanish Ministry of Science and Innovation grant EQC2019-005914-P.
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Fernandez, M.A., Alzayat, H., Jaimes, M.C., Kharraz, Y., Requena, G., Mendez, P. (2022). High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry. In: Ooi, A.T. (eds) Single-Cell Protein Analysis. Methods in Molecular Biology, vol 2386. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1771-7_4
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DOI: https://doi.org/10.1007/978-1-0716-1771-7_4
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