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Immunochemistry Analysis Using Chromogenic Substrates on Tissue Sections

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Single-Cell Protein Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2386))

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Abstract

Immunohistochemistry (IHC) is a highly sensitive protein detection technique developed using the principle of antigen-antibody binding reaction. With immunohistochemistry, it is possible to visualize the abundance, distribution, and localization of proteins in situ. This chapter discusses the standard protocols involved in IHC detection using chromogenic substrates, including pre-treatment of tissues, types of chromogenic substrates, and troubleshooting at various stages of the protocol.

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Author information

Authors and Affiliations

  1. Immunology and Respiratory Research, Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, CT, USA

    Preethi Vijayaraj

Authors
  1. Preethi Vijayaraj
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Correspondence to Preethi Vijayaraj .

Editor information

Editors and Affiliations

  1. Mission Bio, Inc., South San Francisco, CA, USA

    Aik T. Ooi

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Vijayaraj, P. (2022). Immunochemistry Analysis Using Chromogenic Substrates on Tissue Sections. In: Ooi, A.T. (eds) Single-Cell Protein Analysis. Methods in Molecular Biology, vol 2386. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1771-7_1

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  • DOI: https://doi.org/10.1007/978-1-0716-1771-7_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1770-0

  • Online ISBN: 978-1-0716-1771-7

  • eBook Packages: Springer Protocols

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