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Methods to Visualize the Actin Cytoskeleton During Plant Cell Division

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Plant Cell Division

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2382))

Abstract

Cell division in plants consists of separating the mother cell in two daughter cells by the centrifugal growth of a new wall. This process involves the reorganization of the structural elements of the cell, namely the microtubules and actin cytoskeleton which allow the coordination, the orientation, and the progression of mitosis. In addition to its implication in those plant-specific structures, the actin cytoskeleton, in close association with the plasma membrane, exhibits specific patterning at the cortex of the dividing cells, and might act as a signaling component. This review proposes an overview of the techniques available to visualize the actin cytoskeleton in fixed tissues or living cells during division, including electron, fluorescent, and super-resolution microscopy techniques.

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Acknowledgments

We are grateful to the SiCE group (RDP, Lyon, France), in particular Yvon Jaillais and Isabelle Fobis-Loisy; to Oliver Hamant and Jan Traas (RDP, Lyon, France) for comments and discussions. This work was supported by ANRJC/JC INTERPLAY (ANR-16-CE13-0021) and ANR PRC PlantScape (ANR-20-CE13-0026) and a SEED FUND ENS LYON-2020.

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  1. Laboratoire Reproduction et Développement des Plantes, Université de Lyon, UMR5667, ENS de Lyon, UCB Lyon 1, CNRS, INRA, Lyon, France

    Marie-Cécile Caillaud

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  1. Marie-Cécile Caillaud
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Correspondence to Marie-Cécile Caillaud .

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  1. SiCE, Laboratoire Reproduction et Développement des Plantes, ENS Lyon, Lyon, France

    Marie-Cécile Caillaud

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Caillaud, MC. (2022). Methods to Visualize the Actin Cytoskeleton During Plant Cell Division. In: Caillaud, MC. (eds) Plant Cell Division. Methods in Molecular Biology, vol 2382. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1744-1_1

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  • DOI: https://doi.org/10.1007/978-1-0716-1744-1_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1743-4

  • Online ISBN: 978-1-0716-1744-1

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